Matches in SemOpenAlex for { <https://semopenalex.org/work/W2187951265> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2187951265 endingPage "408" @default.
- W2187951265 startingPage "402" @default.
- W2187951265 abstract "Background: Stable dry-state storage of DNA is desirable to minimize required storage space and to reduce electrical and shipping costs. DNA purified from various commercially available dry-state stabilization matrices has been used successfully in downstream molecular applications (e.g., quantitative polymerase chain reaction [qPCR], microarray, and sequence-based genotyping). However, standard DNA storage conditions still include freezing of DNA eluted in aqueous buffers or nuclease-free water. Broad implementation of dry-state, long-term DNA storage requires enhancement of such dry-state DNA stabilization products to control for temperature fluctuations at specimen collection, transit, and storage. This study tested the integrity of genomic DNA subjected to long-term storage on GenTegra™ DNA stabilization matrices (GenTegra LLC, Pleasanton, CA) at extreme conditions, as defined by a 4-year storage period at ambient temperature with an initial incubation for 7 months at 37°C, 56°C, or ambient temperature. Subsequently, purified DNA performance and integrity were measured by qPCR and next-generation sequencing (NGS)-based human leokocyte antigen (HLA) genotyping. Results: High molecular weight genomic DNA samples were recovered from the GenTegra product matrix and exhibited integrity comparable to a highly characterized commercial standard under assessment by qPCR. Samples were genotyped for classical HLA loci using next generation sequencing-based methodolgy on the Roche 454 GS Junior instrument. Amplification efficiency, sequence coverage, and sequence quality were all comparable with those produced from a cell line DNA sequenced as a control. No significant differences were observed in the mean, median, or mode quality scores between samples and controls (p≥0.4). Conclusions: Next generation HLA genotyping was chosen to test the integrity of GenTegra-treated genomic DNA due to the requirment for long sequence reads to genotype the highly polymorphic classical HLA genes. Experimental results demonstrate the efficacy of the GenTegra product as a suitable genomic DNA preservation tool for collection and long-term biobanking of DNA at fluctuating and high temperatures." @default.
- W2187951265 created "2016-06-24" @default.
- W2187951265 creator A5010199055 @default.
- W2187951265 creator A5010282324 @default.
- W2187951265 creator A5021740624 @default.
- W2187951265 creator A5046569059 @default.
- W2187951265 creator A5066398008 @default.
- W2187951265 date "2014-12-01" @default.
- W2187951265 modified "2023-10-15" @default.
- W2187951265 title "DNA Storage under High Temperature Conditions Does Not Affect Performance in Human Leukocyte Antigen Genotyping via Next-Generation Sequencing (DNA Integrity Maintained in Extreme Conditions)" @default.
- W2187951265 cites W1806963385 @default.
- W2187951265 cites W1840573643 @default.
- W2187951265 cites W1965522578 @default.
- W2187951265 cites W1968789418 @default.
- W2187951265 cites W2044844501 @default.
- W2187951265 cites W2059670608 @default.
- W2187951265 cites W2083255580 @default.
- W2187951265 cites W2109086160 @default.
- W2187951265 cites W2119923823 @default.
- W2187951265 cites W2132543522 @default.
- W2187951265 cites W2135460817 @default.
- W2187951265 cites W2142236214 @default.
- W2187951265 cites W2164322381 @default.
- W2187951265 cites W2165308292 @default.
- W2187951265 cites W2404787444 @default.
- W2187951265 cites W4233546182 @default.
- W2187951265 doi "https://doi.org/10.1089/bio.2014.0036" @default.
- W2187951265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4346573" @default.
- W2187951265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25496152" @default.
- W2187951265 hasPublicationYear "2014" @default.
- W2187951265 type Work @default.
- W2187951265 sameAs 2187951265 @default.
- W2187951265 citedByCount "5" @default.
- W2187951265 countsByYear W21879512652017 @default.
- W2187951265 countsByYear W21879512652018 @default.
- W2187951265 countsByYear W21879512652019 @default.
- W2187951265 countsByYear W21879512652020 @default.
- W2187951265 countsByYear W21879512652023 @default.
- W2187951265 crossrefType "journal-article" @default.
- W2187951265 hasAuthorship W2187951265A5010199055 @default.
- W2187951265 hasAuthorship W2187951265A5010282324 @default.
- W2187951265 hasAuthorship W2187951265A5021740624 @default.
- W2187951265 hasAuthorship W2187951265A5046569059 @default.
- W2187951265 hasAuthorship W2187951265A5066398008 @default.
- W2187951265 hasBestOaLocation W21879512652 @default.
- W2187951265 hasConcept C104317684 @default.
- W2187951265 hasConcept C135763542 @default.
- W2187951265 hasConcept C153911025 @default.
- W2187951265 hasConcept C17757408 @default.
- W2187951265 hasConcept C2777271071 @default.
- W2187951265 hasConcept C31467283 @default.
- W2187951265 hasConcept C49105822 @default.
- W2187951265 hasConcept C54355233 @default.
- W2187951265 hasConcept C552990157 @default.
- W2187951265 hasConcept C78063203 @default.
- W2187951265 hasConcept C86803240 @default.
- W2187951265 hasConceptScore W2187951265C104317684 @default.
- W2187951265 hasConceptScore W2187951265C135763542 @default.
- W2187951265 hasConceptScore W2187951265C153911025 @default.
- W2187951265 hasConceptScore W2187951265C17757408 @default.
- W2187951265 hasConceptScore W2187951265C2777271071 @default.
- W2187951265 hasConceptScore W2187951265C31467283 @default.
- W2187951265 hasConceptScore W2187951265C49105822 @default.
- W2187951265 hasConceptScore W2187951265C54355233 @default.
- W2187951265 hasConceptScore W2187951265C552990157 @default.
- W2187951265 hasConceptScore W2187951265C78063203 @default.
- W2187951265 hasConceptScore W2187951265C86803240 @default.
- W2187951265 hasIssue "6" @default.
- W2187951265 hasLocation W21879512651 @default.
- W2187951265 hasLocation W21879512652 @default.
- W2187951265 hasLocation W21879512653 @default.
- W2187951265 hasLocation W21879512654 @default.
- W2187951265 hasOpenAccess W2187951265 @default.
- W2187951265 hasPrimaryLocation W21879512651 @default.
- W2187951265 hasRelatedWork W1966350503 @default.
- W2187951265 hasRelatedWork W1968755513 @default.
- W2187951265 hasRelatedWork W2007427949 @default.
- W2187951265 hasRelatedWork W2152580372 @default.
- W2187951265 hasRelatedWork W2359710606 @default.
- W2187951265 hasRelatedWork W2364958095 @default.
- W2187951265 hasRelatedWork W2368970324 @default.
- W2187951265 hasRelatedWork W2387902386 @default.
- W2187951265 hasRelatedWork W3150161563 @default.
- W2187951265 hasRelatedWork W955585230 @default.
- W2187951265 hasVolume "12" @default.
- W2187951265 isParatext "false" @default.
- W2187951265 isRetracted "false" @default.
- W2187951265 magId "2187951265" @default.
- W2187951265 workType "article" @default.