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- W2187968497 abstract "The black orchid (Coelogyne pandurata Lindley) is an endemic orchid from East Kalimantan, that is characterized by the large size of flowers, with greenish sepals and petals, with a black labellum. Recently, the population of this orchid was decreased and its extinction threatened to be extinct because of overcollecting and/or habitat destruction. The mass propagation of this orchid is urgently needed. Micropropagation of the black orchid was carried out by introducing the Arabidopsis gene Knotted1-like Arabidopsis thaliana (KNAT1) using Agrobacterium tumefaciens. As the plant materials, four-month-old protocorms that were maintained on Vacin and Went (VW) medium were used with 3 replications. The experiment was carried out in three steps: 1) Obtaining the most suitable basic medium (Vacin and Went, VW; New Phalaenopsis, NP; Murashige dan Skoog, MS; and KNUDSON C, KC); 2) Selection of the best phase of developing protocorms for gene transfer; 3) and Genetic transformation of plasmid 35S-KNAT1 and pGreen (empty vector plasmid) using A. tumefaciens strain LBA4404 into orchid protocorms. The results show that the best medium for in vitro culture of the black orchid protocorm is a half strength of MS (1/2 MS) medium that could increase the growth rate of 9.6% protocorms up to phase 6 after 12-week cultivation. But after 12 weeks, the best medium for shoot induction to obtain plantlets is NP medium, so that 11.5% protocorms become plantlets with three leaves. The best condition for gene transfer is five-month-old protocorm. The frequency of genetic transformation using A. tumefaciens is 32.9% for pGreen vektor and 43.2% for p35S-KNAT1. This work is in progress." @default.
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- W2187968497 date "2010-03-01" @default.
- W2187968497 modified "2023-09-24" @default.
- W2187968497 title "Genetic Transformation of the Indonesian Black Orchid (Coelogyn pandurata) through Agrobactrium tumefaciens for Micropropagation" @default.
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