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- W2188023940 abstract "<b><i>Background:</i></b> Downy mildews are a group of microorganisms belonging to the Chromista kingdom that can infect specific plants. When growing on plant tissues these microbes can elicit the expression of pathogenesis-related proteins (PRs), a group of stress-induced proteins frequently described as allergens in many plant species. Our aim was to verify by a proteomic approach whether the allergic reactions experienced by a farmer working in a vineyard infected by <i>Plasmopara viticola</i> (Pv), the etiological agent of downy mildew, are elicited by PRs expressed by the grapevine upon infection or by allergens present in Pv. <b><i>Methods:</i></b> A skin prick test and prick-to-prick test with infected field grapevine leaves and control leaves were carried out. Field leaves and ad hoc Pv-inoculated leaves were compared by SDS-PAGE and IgE-immunoblotting with extracts from control leaves and Pv sporangia. IgE-binding proteins were further separated by two-dimensional electrophoresis and the positive spots analyzed by nanoHPLC-Chip and tandem mass spectrometry (MS/MS) for identification. <b><i>Results:</i></b> Only infected leaves showed IgE-binding protein bands at 42 and 36 kDa. This agreed with the positive skin prick test experienced by the patient only with the infected leaves extract. Two-dimensional electrophoresis followed by MS/MS analysis led to the identification of PR-2 (β-1,3-glucanase) and harpin-binding protein 1 as putative allergens, the latter having never been reported before. <b><i>Conclusion:</i></b> The results indicate that Pv infection might represent a new source of plant allergens." @default.
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- W2188023940 date "2015-01-01" @default.
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- W2188023940 title "Grapevine Downy Mildew <b><i>Plasmopara viticola</i></b> Infection Elicits the Expression of Allergenic Pathogenesis-Related Proteins" @default.
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- W2188023940 doi "https://doi.org/10.1159/000441792" @default.
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