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- W2188042534 abstract "This work provides the first description of a highly effective and specific siRNA-based inhibitor for the potential oncogene hEAG1 (human ether a go-go channel), which proved to be effective on RNA, protein and activity as well as functional level (proliferation, modulation of expression of target genes). Therefore this study created a basis for the development of an EAG1-specific cancer therapy. The zinc finger transcription factor MAZ was identified as a downstream target of EAG1 by means of two independent methods, the subtractive hybridization and RNA interference. A definite time course of MAZ induction was observed in three model cell lines when EAG1 expression was shut down by RNAi. Binding sites for this transcription factor have been detected in promoters of cancer related genes like c-MYC (Kabilova et al., 2006) and TERT (Cong et al., 2002) regulating their expression. This prompted the investigation of the time courses of these two genes upon EAG1-siRNA treatment resulting in a significant repression of the amount of their RNA. Moreover, by applying new controls and an optimization strategy this work represents a significant contribution to the research field of RNAi. Several genes responsible for the physiological function of EAG1 were detected by subtractive hybridization and verified by quantitative Real-Time-PCR (RACK1, MMP2, Fibronektin, GAPDH). First evidence for the existance of multiple forms of EAG1 in cancer cell lines differing from the form present in brain were found by sequencing.Additionally, EAG RNA has been localized in several organs outside of the brain in mice and has been shown to be modulated in peripheral organs, but not in brain, by triggering a sterile inflammation by intraperitoneal injection of Lipopolysaccharides (LPS)." @default.
- W2188042534 created "2016-06-24" @default.
- W2188042534 creator A5021839235 @default.
- W2188042534 date "2006-08-02" @default.
- W2188042534 modified "2023-09-24" @default.
- W2188042534 title "Konsequenzen der Expression des Ether à go-go Kaliumkanals" @default.
- W2188042534 cites W103701665 @default.
- W2188042534 cites W138847678 @default.
- W2188042534 cites W1498322573 @default.
- W2188042534 cites W1503431173 @default.
- W2188042534 cites W1517480469 @default.
- W2188042534 cites W1565572133 @default.
- W2188042534 cites W1570113205 @default.
- W2188042534 cites W1573961429 @default.
- W2188042534 cites W1577950031 @default.
- W2188042534 cites W1585481509 @default.
- W2188042534 cites W1586462640 @default.
- W2188042534 cites W1590562161 @default.
- W2188042534 cites W1611419331 @default.
- W2188042534 cites W164603943 @default.
- W2188042534 cites W1718849794 @default.
- W2188042534 cites W1777367897 @default.
- W2188042534 cites W1877364173 @default.
- W2188042534 cites W1879167427 @default.
- W2188042534 cites W1929670121 @default.
- W2188042534 cites W1934871911 @default.
- W2188042534 cites W1965618147 @default.
- W2188042534 cites W1965934296 @default.
- W2188042534 cites W1966542709 @default.
- W2188042534 cites W1969773497 @default.
- W2188042534 cites W1970120474 @default.
- W2188042534 cites W1970156673 @default.
- W2188042534 cites W1971741728 @default.
- W2188042534 cites W1975058909 @default.
- W2188042534 cites W1976331921 @default.
- W2188042534 cites W1976352894 @default.
- W2188042534 cites W1976551644 @default.
- W2188042534 cites W1978591675 @default.
- W2188042534 cites W1979146520 @default.
- W2188042534 cites W1979869118 @default.
- W2188042534 cites W1979870127 @default.
- W2188042534 cites W1980195431 @default.
- W2188042534 cites W1981014385 @default.
- W2188042534 cites W1981716019 @default.
- W2188042534 cites W1981891742 @default.
- W2188042534 cites W1983204782 @default.
- W2188042534 cites W1984044444 @default.
- W2188042534 cites W1984159955 @default.
- W2188042534 cites W1985586890 @default.
- W2188042534 cites W1986974750 @default.
- W2188042534 cites W1988036288 @default.
- W2188042534 cites W1989076816 @default.
- W2188042534 cites W1989318364 @default.
- W2188042534 cites W1990858735 @default.
- W2188042534 cites W1994359575 @default.
- W2188042534 cites W1995996876 @default.
- W2188042534 cites W1996163490 @default.
- W2188042534 cites W1997071909 @default.
- W2188042534 cites W1999073594 @default.
- W2188042534 cites W2000253969 @default.
- W2188042534 cites W2001927863 @default.
- W2188042534 cites W2004812096 @default.
- W2188042534 cites W2004844570 @default.
- W2188042534 cites W2009667219 @default.
- W2188042534 cites W2010603221 @default.
- W2188042534 cites W2010817360 @default.
- W2188042534 cites W2012739573 @default.
- W2188042534 cites W2014489011 @default.
- W2188042534 cites W2014791035 @default.
- W2188042534 cites W2016704876 @default.
- W2188042534 cites W2016710415 @default.
- W2188042534 cites W2018424508 @default.
- W2188042534 cites W2018556537 @default.
- W2188042534 cites W2019410656 @default.
- W2188042534 cites W2019411198 @default.
- W2188042534 cites W2020259516 @default.
- W2188042534 cites W2020414117 @default.
- W2188042534 cites W2021951737 @default.
- W2188042534 cites W2022422805 @default.
- W2188042534 cites W2023538913 @default.
- W2188042534 cites W2024450126 @default.
- W2188042534 cites W2025793732 @default.
- W2188042534 cites W2029179317 @default.
- W2188042534 cites W2032118018 @default.
- W2188042534 cites W2037606662 @default.
- W2188042534 cites W2043696829 @default.
- W2188042534 cites W2044296969 @default.
- W2188042534 cites W2044326283 @default.
- W2188042534 cites W2048994896 @default.
- W2188042534 cites W2049274590 @default.
- W2188042534 cites W2050066317 @default.
- W2188042534 cites W2050466704 @default.
- W2188042534 cites W2051292461 @default.
- W2188042534 cites W2053268418 @default.
- W2188042534 cites W2054757478 @default.
- W2188042534 cites W2056892651 @default.
- W2188042534 cites W2058386795 @default.
- W2188042534 cites W2061846436 @default.
- W2188042534 cites W2063176176 @default.
- W2188042534 cites W2065874620 @default.