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- W2188124323 abstract "Studies were conducted to compare the metabolism of 75Se-selenomethionine, 75Se-selenite,75Se-selenate and 35S-sulfate by rumen microorganisms in vitro. There was a gradual incorporation of 35sulfur activity into the TCA-insoluble fraction of the rumen fluid which could be inhibited by excess sulfate. The incorporation of 35sulfur into methionine was confirmed by paper and ion exchange chromatography. There was a gradual incorporation of 75Se-selenomethionine into the TCA-insoluble fraction of the rumen fluid which could be inhibited by excess methionine;Pronase released most of the activity from the TCA-insoluble fraction, approximately 60% of which was identified as 75Se-selenomethionine by paper and ion exchange chromatography. Radioactivity added to the rumen fluid as 75Se-selenite and 75Se-selenate was rapidly incorporated into the TCA-insoluble fraction. However, most of the activity added as inorganic selenium was apparently loosely bound and could be removed by dialysis when reduced glutathione was in the dialysis bath; the binding of selenite was not enzyme dependent. The incorporation of inorganic selenium into the TCA-insoluble fraction was not inhibited by excess sulfate, and very little or none of the activity added to the rumen fluid as 75Se-selenite or 75Se-selenate was incorporated into 75Se-selenomethionine." @default.
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- W2188124323 date "1968-01-01" @default.
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- W2188124323 title "Metabolism of Se-Selenite, Se-Selenate, Se-Selenomethionine and S-Sulfate by Rumen Microorganisms" @default.
- W2188124323 doi "https://doi.org/10.2527/jas1968.272497x" @default.
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