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• W2188233890 abstract "Ducrosia. Anethifolia (DC.) is an important medicinal herb belonging to the family Apiaceae. The present study was undertaken to study the effect of different concentrations of plant growth regulators and various parts of D. anethifolia on callus induction and regeneration. Various explants: leaf, node, stem, root, and petiole taken from the actively growing plants. Explants were cultured on Murashig and Skoog (MS) medium supplemented with 2-4- Dichlorophenoxyacetic acid (2, 4-D; 1–4 mg.l -1 ) or α- Naphatalenacetic acid (NAA; 1-4 mg.l -1 ) alone or in combination with N6-Benzil adenine (BA; 0-1 mg.l -1 ) or Kinetin; (KIN 0-1 mg.l -1 ) for callus induction. The best response (100%) was observed in stems on MS medium with (2 mg.l -1 NAA and 1 mg.l -1 BA) and on MS medium containing (3 mg.l -1 NAA and 1 mg.l -1 BA) with stem, leaf and node. These calli were transferred to MS medium supplemented with various concentrations of BA (1– 2 mg.l -1 ) or KIN (1–2 mg.l -1 ) alone or in combination with indole 3-aceticacid (IAA: 0.1–0.5 mg.l -1 ) or NAA (0.1–0.5 mg.l -1 ) or indole butyric acid (IBA: 0.1–0.5 mg.l -1 ) for shoot regeneration. Calli derived from Stem segment Showed significantly highest frequency of shoot regeneration. Rooting of in vitro raised shoots was mostly induced on Quarter strength MS (100%). Regenerated plants established in original habitat. This model procedure can be used for as a source for the isolation of secondary metabolites from D. anethifolia." @default.
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• W2188233890 date "2014-01-01" @default.
• W2188233890 modified "2023-09-27" @default.
• W2188233890 title "Original Contribution ESTABLISHMENT OF AN EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION SYSTEM IN DUCROSIA ANETHIFOLIA (DC.) AN IMPORTANT MEDICINAL PLANT" @default.
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