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- W2188250584 abstract "Primers specific toconserved andvariable regions inthe16SrRNAsequencewere selected fromthepartially sequenced 16SrRNAgenes ofNeisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, andStaphylococcus epidermidis. ThePCRassaywas divided into twoDNA amplifications. Thefirst resulted ina general bacterial amplicon, andthesecond resulted ina species-specific amplicon. Thehigh specificity ofthePCRassaywas documented after testing bacteria of28different species (133strains). Atotal of304clinical cerebrospinal fluid samples, including 125samples frompatients withbacterial meningitis, were assayed toinvestigate thediagnostic sensitivity andspecificity forbacterial meningitis. Theassayshowedhigh sensitivity (0.94) andspecificity (0.96) withtheclinical samples, although some false results wereobtained, the reasonsforwhichare discussed. Withagarosegelelectrophoresis fordetection ofthePCR products, the detection limit formeningococci incerebrospinal fluid was 3 x 102CFU/ml." @default.
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- W2188250584 date "1994-01-01" @default.
- W2188250584 modified "2023-09-23" @default.
- W2188250584 title "Detection ofBacterial DNA inCerebrospinal Fluid byan Assay forSimultaneous Detection ofNeisseria meningitidis, Haemophilus influenzae, andStreptococci Using a Seminested PCRStrategy" @default.
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