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- W2188291296 abstract "Two antimicrobial peptides namely bovine tracheal a ntimicrobial peptide (TAP) and bovine neutrophil β-defensin 4 (BNBD4) were investigated in crossbred Frisian and Egyptian native cattle. TAP and BNBD4 were tested for reaction with cDNA of various cattle tissues such as liver, lung, trachea, intestine, mammary gland, colon and blood using PCR. TAP and BNBD4 were positive in all tissues of the native cattle whereas in crossbr ed Frisian only the trachea PCR- product sequence was positive with both. NCBI-Blast analysis of TAP and BNBD4 sequences showed 89% and 92% alignment between crossbred Frisian and native catt le, respectively. Blast analysis of TAP PCRproduct sequences of both native and crossbred Fris ian cattle with GenBank published sequences revealed sequence homology between both native and crossbred Frisian cattle and Bos taurus TAP mRNA. Analysis also revealed homology (90%) between crossbred Frisian TAP and Bos taurus lingual antimicrobial peptide (LAP), whereas native cattle TAP was homologous (86%) to Bubalus bubalis LAP. Blast analysis of BNBD4 PCR-products s equence revealed sequence homology between both native and crossbred Frisian cattle an d Bos taurus BNBD4 mRNA. Analysis also revealed homology with Bubalus bubalis enteric beta -defensin preproprotein mRNA, however the native cattle showed higher homology (128 nucleotid e shared) than the crossbred Frisian where only 62 nucleotides were shared. The presence of TA P and BNBD4 in all tested tissues of native cattle may reflect a higher tendency towards diseas e resistance in native cattle compared to the crossbred Frisian ones. Further studies on the expr ession of these genes in different organs of the native cattle will shed more light on their disease resistance." @default.
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- W2188291296 date "2008-01-01" @default.
- W2188291296 modified "2023-09-27" @default.
- W2188291296 title "Identification and sequence analysis of two antimic robial peptides in Egyptian native and crossbred frisian c attle" @default.
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