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- W2188345943 abstract "Lactobacillus acidophilus was isolated from fermented millet, Eleusine coracana. It was characterized using several biochemical tests. This strain was found to be homofermentative, slime forming and β-galactosidase producer. Recovery and characterization of β-galactosidase were studied at laboratory scale. Since the enzyme was intracellular various cell lysis methods were studied to achieve maximum enzyme release from cell fragments. Homogenization at a pressure of 2000 psig two passes showed an enzyme release of 2050 U/mL and was found the best method for cell lysis. The cell extract was purified using ultrafiltration and gel permeation chromatography. Specific activity and fold purification of β-galactosidase was found to be 568.61 and 21.2, respectively. Kinetic parameters were determined using ONPG (o-nitrophenyl galactopyranoside) as a substrate. Michaeli’s Menten equation was found to fit the reaction of ONPG using β-galactosidase from L. acidophilus. Vmax and Km were calculated from the Lineweaver Burk plot. Vmax was found to be 4.94 min -1 and Km to be 0.11 mM. Using gel permeation chromatography, the molecular weight was found to be in the range of 450-500 kDa." @default.
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- W2188345943 date "2006-04-01" @default.
- W2188345943 modified "2023-09-27" @default.
- W2188345943 title "β-Galactosidase from Lactobacillus acidophilus isolated from fermented ragi (Eleusine coracana)" @default.
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