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- W2188488394 abstract "Cl − efflux induces depolarization and contraction of smooth muscle cells. This study was undertaken to explore the role of Cl − channels in endothelin-1 (ET-1)-induced contraction in rabbit basilar artery. Male New Zealand White rabbits ( n = 26), weighing 1.8–2.5 kg, were euthanized by an overdose of pentobarbital. The basilar arteries were removed for isometric tension recording. ET-1 produced a concentration-dependent contraction of the rabbit basilar artery in the normal Cl − Krebs-Henseleit bicarbonate buffer (123 mM Cl − ). The ET-1-induced contraction was reduced by the following manipulations: 1) inhibition of Na + -K + -2Cl − cotransporter with bumetanide (3 × 10 −5 and 10 −4 M), 2) bicarbonate-free solution to disable Cl − /HCO[Formula: see text] exchanger, and 3) preincubation of rings with the Cl − channel blockers niflumic acid, 5-nitro-2-(3-phenylpropylamino)benzoic acid, and indanyloxyacetic acid 94. The ET-1-induced contraction was enhanced by substitution of extracellular Cl − (10 mM) with methanesulfonic acid (113 mM). Cl − channels are involved in ET-1-induced contraction in the rabbit basilar artery." @default.
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- W2188488394 date "2001-11-01" @default.
- W2188488394 modified "2023-09-28" @default.
- W2188488394 title "Role of Cl− current in endothelin-1-induced contraction in rabbit basilar artery" @default.
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- W2188488394 doi "https://doi.org/10.1152/ajpheart.2001.281.5.h2159" @default.
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