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- W2188571156 abstract "The increase in antibiotic resistance among Gram-negative bacteria is a notable example of how bacteria can procure, maintain and express new genetic information that can confer resistance to one or several antibiotics. The emergence of multidrug resistant (MDR) Salmonella has become a serious problem. Our study was done to investigate the role of plasmid as a factor mediating such resistance pattern. This study was done on 40 Salmonella isolates from different food products. Antibiogram was done by Kirby Bauer disc diffusion method and the minimum inhibitory concentration (MIC) was determined using the microtiter broth dilution method. MDR isolates (23/40) were confirmed and identified to species level using API 20E. Plasmid extraction was done for MDR isolates by alkaline lysis. It was revealed that 60.86% (14/23) of MDR isolates were found to harbour plasmids. To demonstrate the role of plasmid in resistance, Salmonella isolates were subjected to plasmid curing using sodium dodecyl sulfate (SDS). The present study has investigated the degree of similarity between five MDR isolates on molecular basis using random amplified polymorphic DNA (RAPD) technique, in which a total of 5 primers were used showing good discriminatory power for all five isolates. The dendrogram showed a common lineage among all five isolates. Finally 16S ribosomal DNA sequencing has been performed for one Salmonella isolate." @default.
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- W2188571156 date "2012-01-01" @default.
- W2188571156 modified "2023-09-23" @default.
- W2188571156 title "Plasmid Encoding Antimicrobial Resistance among Environmental Salmonella Species and Molecular Characterization Using Random Amplified Polymorphic DNA Analysis" @default.
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