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- W2188617967 abstract "A special swinging bucket rotor has been constructed which produces fixed and stained dispersions of cells obtained from dilute suspensions on conventional microscope slides. In each of eight sample chambers 12,500-250,000 cells are sedi- men ted onto the slide. Then 0.1 ml of a 4% glutaraldehyde, 12% dimethl sulfox- ide fixing solution is layered over the cells and the centrifuge is restarted. The application of the centrifugal field during fixation flattens the cells and presuma- hlminimizes cell losses from the slide. After 45 mm the bucket is disassemhle(l and the cells are benzidine-stained for hemoglobin and then the slide is post- fixed with ethanol, after which the cells are stained with Giemsa, dehydrated through the ethanols, stained with Sudan black and then mounted without air- drying at any intermediate step. Cover slip preparations, air-di'ied from xylene, are utilized for scanning electron microscopy. Quantitative cell counts of known concentrations of blood cells have demonstrated that 89.2w of the cells am'e re- covered in the mounted preparation. A reconstruction experiment utilizing van- otis synthetic mixtures of human and tadpole blood cells established that there is no selection artifact in time ratio of these cells recovered on the entire slide nor any artifacts due to nondiscriminate cell placement. Cell size determinations of glutaraldehyde-fixed erythrocytes were performed in an attempt to determine" @default.
- W2188617967 created "2016-06-24" @default.
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- W2188617967 date "1971-01-01" @default.
- W2188617967 modified "2023-09-27" @default.
- W2188617967 title "I. A QUANTITATIVE TECHNIQUE FOR THE PREPARATION OF GLUTARALDEHYDE-FIXED CELLS FOR THE LIGHT AND SCANNING ELECTRON MICROSCOPE1" @default.
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