Matches in SemOpenAlex for { <https://semopenalex.org/work/W2188779811> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2188779811 abstract "Rubisco large subunit methyltransferase (PsLSMT) is a SET domain protein responsible for the trimethylation of Lys-14 in the large subunit of Rubisco. The polypeptide substrate specificity determinants for pea Rubisco large subunit methyltransferase were investigated using a fusion protein construct between the first 23 amino acids from the large subunit of Rubisco and human carbonic anhydrase II. A total of 40 conservative and non-conservative amino acid substitutions flanking the target Lys-14 methylation site (positions P3 to P3) were engineered in the fusion protein. The catalytic efficiency (kcat/ Km )o fPsLSMT was determined using each of the substitutions and a polypeptide consensus recognition sequence deduced from the results. The consensus sequence, represented by X-(Gly/Ser)-(Phe/Tyr)-Lys-(Ala/Lys/Arg)-(Gly/Ser)-, where X is any residue, Lys is the methylation site, and is any aromatic or hydrophobic residue, was used to predict potential alternative substrates for PsLSMT. Four chloroplast-localized proteins were identified including -tocopherol methyltransferase (-TMT). In vitro methylation assays using PsLSMT and a bacterially expressed form of -TMT from Perilla frutescens confirmed recognition and methylation of -TMT by PsLSMT in vitro. RNA interference-mediated knockdown of the PsLSMT homologue (NtLSMT) in transgenic tobacco plants resulted in a 2-fold decrease of -tocopherol, the product of -TMT. The results demonstrate the efficacy of consensus sequence-driven identification of alternative substrates for PsLSMT as well as identification of functional attributes of protein methylation catalyzed by LSMT." @default.
- W2188779811 created "2016-06-24" @default.
- W2188779811 creator A5024424468 @default.
- W2188779811 creator A5044356587 @default.
- W2188779811 creator A5056894690 @default.
- W2188779811 creator A5070609079 @default.
- W2188779811 creator A5089068083 @default.
- W2188779811 date "2007-01-01" @default.
- W2188779811 modified "2023-09-27" @default.
- W2188779811 title "Fromthe ‡ DepartmentofHorticulture,PlantPhysiology/Biochemistry/MolecularBiologyProgram,UniversityofKentucky, Lexington,Kentucky40546-0312andthe § DepartmentofPlantSciences,UniversityofTennessee,Knoxville, Tennessee 37996-4561" @default.
- W2188779811 cites W2088201199 @default.
- W2188779811 cites W2129098267 @default.
- W2188779811 hasPublicationYear "2007" @default.
- W2188779811 type Work @default.
- W2188779811 sameAs 2188779811 @default.
- W2188779811 citedByCount "0" @default.
- W2188779811 crossrefType "journal-article" @default.
- W2188779811 hasAuthorship W2188779811A5024424468 @default.
- W2188779811 hasAuthorship W2188779811A5044356587 @default.
- W2188779811 hasAuthorship W2188779811A5056894690 @default.
- W2188779811 hasAuthorship W2188779811A5070609079 @default.
- W2188779811 hasAuthorship W2188779811A5089068083 @default.
- W2188779811 hasConcept C104292427 @default.
- W2188779811 hasConcept C104317684 @default.
- W2188779811 hasConcept C123894998 @default.
- W2188779811 hasConcept C153911025 @default.
- W2188779811 hasConcept C167625842 @default.
- W2188779811 hasConcept C181199279 @default.
- W2188779811 hasConcept C185592680 @default.
- W2188779811 hasConcept C2776809771 @default.
- W2188779811 hasConcept C33288867 @default.
- W2188779811 hasConcept C40767141 @default.
- W2188779811 hasConcept C50212798 @default.
- W2188779811 hasConcept C553450214 @default.
- W2188779811 hasConcept C55493867 @default.
- W2188779811 hasConcept C86803240 @default.
- W2188779811 hasConcept C91965660 @default.
- W2188779811 hasConceptScore W2188779811C104292427 @default.
- W2188779811 hasConceptScore W2188779811C104317684 @default.
- W2188779811 hasConceptScore W2188779811C123894998 @default.
- W2188779811 hasConceptScore W2188779811C153911025 @default.
- W2188779811 hasConceptScore W2188779811C167625842 @default.
- W2188779811 hasConceptScore W2188779811C181199279 @default.
- W2188779811 hasConceptScore W2188779811C185592680 @default.
- W2188779811 hasConceptScore W2188779811C2776809771 @default.
- W2188779811 hasConceptScore W2188779811C33288867 @default.
- W2188779811 hasConceptScore W2188779811C40767141 @default.
- W2188779811 hasConceptScore W2188779811C50212798 @default.
- W2188779811 hasConceptScore W2188779811C553450214 @default.
- W2188779811 hasConceptScore W2188779811C55493867 @default.
- W2188779811 hasConceptScore W2188779811C86803240 @default.
- W2188779811 hasConceptScore W2188779811C91965660 @default.
- W2188779811 hasLocation W21887798111 @default.
- W2188779811 hasOpenAccess W2188779811 @default.
- W2188779811 hasPrimaryLocation W21887798111 @default.
- W2188779811 hasRelatedWork W1479783860 @default.
- W2188779811 hasRelatedWork W1491606618 @default.
- W2188779811 hasRelatedWork W1529224585 @default.
- W2188779811 hasRelatedWork W1587109379 @default.
- W2188779811 hasRelatedWork W1593971807 @default.
- W2188779811 hasRelatedWork W1824296766 @default.
- W2188779811 hasRelatedWork W1834615885 @default.
- W2188779811 hasRelatedWork W1969973679 @default.
- W2188779811 hasRelatedWork W2011982205 @default.
- W2188779811 hasRelatedWork W2012719716 @default.
- W2188779811 hasRelatedWork W2038869180 @default.
- W2188779811 hasRelatedWork W2051877397 @default.
- W2188779811 hasRelatedWork W2064034713 @default.
- W2188779811 hasRelatedWork W2073129019 @default.
- W2188779811 hasRelatedWork W2088836184 @default.
- W2188779811 hasRelatedWork W2092147306 @default.
- W2188779811 hasRelatedWork W2092306581 @default.
- W2188779811 hasRelatedWork W2468047979 @default.
- W2188779811 hasRelatedWork W279033900 @default.
- W2188779811 hasRelatedWork W344785125 @default.
- W2188779811 isParatext "false" @default.
- W2188779811 isRetracted "false" @default.
- W2188779811 magId "2188779811" @default.
- W2188779811 workType "article" @default.