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- W2188806670 abstract "Methylation patterns ofChlamydomonas chlor-oplast DNAs(chIDNAs) wereexamined inthevegetative, gametic, andzygotic stages ofthelife cycle. Restriction endo- nuclease fragment patterns produced byEcoRI, BamHI, Hpa II, andMspIwerecompared; thelast twocleave DNAatthe sequence CC-C-C, butHpaIIisblocked byprior methylation oftheinternal cytidine whereas MspIisnot. chlDNAs from vegetative cells ofbothmating types showed noevidence of methylation atC-C-CG-. Gametic mt+chlDNAwasheavily methylated atC-C-C-, whereas thehomologous chlDNA from mt-gametes showed veryslight methylation atC-C-C-G. Methylation ofadditional sites inchlDNA frommt+gametes butnotfrommt-gametes wasshownbyblockage ofsome EcoRIandBamHIsites that werecleaved inthechlDNA from vegetative cells. chlDNAfrom6-hr zygotes wasmuchmore methylated than gametic mt+DNA,asshown byits almost total resistance tocleavage byall four restriction enzymes. These findings support andextend previous evidence that chlDNA of mt+cells ismethylated during gametogenesis andthat further methylation occurs after gametic fusion intheyoungzy- gotes. Themethylation andrestriction ofchloroplast DNA(chlDNA) hasbeen shown (1-4) toplay adecisive role intheregulation ofmaternal inheritance ofthechloroplast genomein Chlamydomonas. Inthis paper, themethylation patterns of chIDNAs purified fromvegetative cells andgametes ofboth mating types andfromzygotes have beencompared with the useoffour restriction endonucleases: HpaII, MspI,EcoRI, and BamHI. HpaIIandMspIboth cleave DNAattheC-C-G-G se-" @default.
- W2188806670 created "2016-06-24" @default.
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- W2188806670 date "1979-01-01" @default.
- W2188806670 modified "2023-09-27" @default.
- W2188806670 title "Methylation ofchloroplast DNAsinthelife cycle ofChlamydomonas (maternal inheritance/DNA methylation/restriction" @default.
- W2188806670 hasPublicationYear "1979" @default.
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