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- W2188889410 abstract "Liquid chromatography-electrospray-mass spectrometry (LC-ES-MS) was employed to analyze five biologically active components (sennoside A and B, rhein, emodin and aloe-emodin) in rhubarb. The separation was achieved on a C18 column using gradient elution. Negative ion detection mode was utilized for ES-MS detection. For sennosides, [M-H](superscript -) and a fragmented ion (m/z=386) were detected. In addition to [M-H](superscript -), [M-COOH](superscript -) was observed for rhein. Only one major ion, [M-H](superscript -), was determined for aloe-emodin and emodin. A post-column splitter was employed to enhance the sensitivity of sennoside measurement. Extracted ion monitoring was applied for quantitative analysis. Satisfactory linearity (r^2=0.99) of each compound was determined. In addition, the content of these five active components in various rhubarb samples were examined by this newly developed LC-ES-MS." @default.
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- W2188889410 date "2003-03-01" @default.
- W2188889410 modified "2023-09-26" @default.
- W2188889410 title "Analysis of Rhubarb by Liquid Chromatography-Electrospray-Mass Spectrometry" @default.
- W2188889410 doi "https://doi.org/10.6180/jase.2003.6.1.04" @default.
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