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- W2188916512 abstract "A Novel Reversed phase High performance liquid chromatography method has been developed and validated for the estimation of Prulifloxacin. A simple, precise, specific, sensitive and accurate Reversed phase HPLC method was developed and validated as per the ICH guidelines for the quantitative determination of Prulifloxacin in bulk and pharmaceutical dosage forms. Isocratic chromatography was performed on Inertsil ODS C18 column (250mm ×4.6mmx 5 µm) at ambient temperature with phosphate buffer: Acetonitrile 30:70%v/v as a mobile phase, pH 3.5 adjusted with 0.1M ortho phosphoric acid at a flow rate is 1.0 ml/min. The eluent detection was carried out by using photodiode array detector at 278 nm. The retention time of Prulifloxacin was 2.764 min. The linearity was observed from 60-180µg/ml with r 2 =0.999. The limit of detection and limit of quantitation were found to be 0.84µg/ml and 1.8µg/ml respectively. The Statistical analysis proves that the method was found to be simple, selective, reliable, accurate and reproducible for successfully used for routine quality control analysis of Prulifloxacin." @default.
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- W2188916512 date "2012-01-01" @default.
- W2188916512 modified "2023-09-27" @default.
- W2188916512 title "DEVELOPMENT AND VALIDATION OF NOVEL ISOCRATIC RP-HPLC METHOD FOR THE ESTIMATION OF PRULIFLOXACIN IN BULK AND PHARMACEUTICAL DOSAGE FORMS" @default.
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