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• W2189019715 abstract "Trichoderma harzianum strain T-22 (T22) has the ability of enhancing root growth and plant development. The aim of this research is to explain the biochemical basis of the direct plant-growth-promoting activity of T22. Seven days after the transfer to root-inducing medium, in vitro-cultured shoots of GiSeLa6 ® (Prunus cerasus × P. canescens), an important Prunus rootstock for sweet and sour cherry cultivars, were inoculated with T22. Root and shoot growth were significantly affected by T22 (+76 and +61%, respectively). Indole-3-acetic acid (IAA), transzeatin riboside (t-ZR) and dihydrozeatin riboside (DHZR) were analyzed by HPLC/MS. Acidification of the medium by plant, T22, and plant + T22 were assessed, whereas root morphological changes were observed by light and epifluorescence microscopic analysis. Nine days after inoculation, the levels of indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), dihydrozeatin riboside (DHZR), gibberellic acid (GA3) and abscisic acid (ABA) were analyzed by high performance liquid chromatography coupled with mass spectrometry. The results showed that after T22-inoculation, IAA and GA3 significantly increased in both leaves (+49 and +71%, respectively) and roots (+40 and +143%, respectively) whereas t-ZR decreased (-51% in leaves and -37% in roots). Changes in DHZR were observed in T22-inoculated roots (-32%) but not in leaves, whereas the levels of ABA did not differ between the two treatments. Root activity determined a decline of medium acidity, and this effect was more marked in T22-inoculated plants (up to pH 4). Microscopic analysis revealed changes in root cell wall suberification in the exoderm and endoderm, with an increase in suberized cellular layers from 1 to 2-3, and an enhancement of cell wall epifluorescence. All these T22-induced changes promote rooting and shoot growth, and they could increase plant survival during the acclimatisation phase of nursery processes." @default.
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• W2189019715 date "2012-10-01" @default.
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• W2189019715 title "BIOCHEMICAL AND MORPHOLOGICAL CHANGES IN MICROPROPAGATED SHOOTS OF GISELA6® (PRUNUS SPP.) ROOTSTOCK INOCULATED WITH TRICHODERMA HARZIANUM STRAIN T-22" @default.
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• W2189019715 doi "https://doi.org/10.17660/actahortic.2012.961.64" @default.
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