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- W2189093445 abstract "We present an approach to the construction of an overlapping restriction fragment map of a single human chromosome. A genomic cosmid library genome was constructed from a mouse-human hybrid cell line containing chromosome 17 as its only human genetic component. Cosmids containing human inserts were isolated by hybridisation to a human Alu sequence. DNAs from ninety-six randomly chosen cosmids were digested with either EcoRI or HindIII, end-labelled with 35SdATP and analysed using agarose gel electrophoresis. Comparison of the restriction fragment patterns revealed two pairs of overlapping clones, that were confirmed by crosshybridisation of the overlapping fragments. The two pairs of cosmids both mapped to human chromosome 17, as shown by hyb ridisation to a panel of somatic cell hybrids. These data demonstrate that the generationof an overlapping cosmid map along a human chromosome is feasible, representing an intermediate step towards the complete sequencingof a human chromosome." @default.
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- W2189093445 date "1987-01-01" @default.
- W2189093445 modified "2023-09-26" @default.
- W2189093445 title "Progress towards construction ofatotal restriction fragnent mapofahumanchromosome" @default.
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