Matches in SemOpenAlex for { <https://semopenalex.org/work/W2189173652> ?p ?o ?g. }
- W2189173652 endingPage "128" @default.
- W2189173652 startingPage "115" @default.
- W2189173652 abstract "AbstractNeuronal and glial cell cultures are important parts of neuroscience laboratories. Because the human brain is the least accessible organ, experimental studies on brain cell cultures provide valuable information. The ability to experiment on real neurons and glial cells in a controlled environment under specific conditions is essential to understand cellular and molecular mechanisms in the brain during development and in disease. Isolation and culture of neurons and glial cells is tricky but very important techniques for neuroscientists. These cells are very fragile, and primary culture of brain cells can be challenging. This chapter describes step-by-step protocols for the isolation and enrichment of different cell types from human brain tissue, and the optimal conditions needed to achieve viable and healthy neuronal and glial cell cultures. Immunocytochemical staining protocols for the brain cells are described with illustrations.KeywordsHumanFetalNeuronsAstrocytesMicrogliaHIV" @default.
- W2189173652 created "2016-06-24" @default.
- W2189173652 creator A5031790890 @default.
- W2189173652 creator A5042126168 @default.
- W2189173652 date "2013-09-30" @default.
- W2189173652 modified "2023-09-27" @default.
- W2189173652 title "Isolation and Culture of Human Neurons, Microglia, and Astrocytes" @default.
- W2189173652 cites W1506970858 @default.
- W2189173652 cites W1519306389 @default.
- W2189173652 cites W1539836277 @default.
- W2189173652 cites W1936351921 @default.
- W2189173652 cites W1976305268 @default.
- W2189173652 cites W1976390944 @default.
- W2189173652 cites W1977994578 @default.
- W2189173652 cites W1978937475 @default.
- W2189173652 cites W1980135039 @default.
- W2189173652 cites W1984550437 @default.
- W2189173652 cites W1987854862 @default.
- W2189173652 cites W1988189150 @default.
- W2189173652 cites W1988664454 @default.
- W2189173652 cites W1997726546 @default.
- W2189173652 cites W1999350895 @default.
- W2189173652 cites W1999881215 @default.
- W2189173652 cites W2002167956 @default.
- W2189173652 cites W2004997979 @default.
- W2189173652 cites W2013290394 @default.
- W2189173652 cites W2013737464 @default.
- W2189173652 cites W2016964866 @default.
- W2189173652 cites W2019248029 @default.
- W2189173652 cites W2026211597 @default.
- W2189173652 cites W2041234985 @default.
- W2189173652 cites W2041476640 @default.
- W2189173652 cites W2044714779 @default.
- W2189173652 cites W2045070017 @default.
- W2189173652 cites W2053180614 @default.
- W2189173652 cites W2054450876 @default.
- W2189173652 cites W2061001694 @default.
- W2189173652 cites W2062268073 @default.
- W2189173652 cites W2064981396 @default.
- W2189173652 cites W2070772192 @default.
- W2189173652 cites W2074298460 @default.
- W2189173652 cites W2081416052 @default.
- W2189173652 cites W2086419987 @default.
- W2189173652 cites W2087874796 @default.
- W2189173652 cites W2089416663 @default.
- W2189173652 cites W2097602995 @default.
- W2189173652 cites W2103575089 @default.
- W2189173652 cites W2105631032 @default.
- W2189173652 cites W2114324710 @default.
- W2189173652 cites W2115425483 @default.
- W2189173652 cites W2130653534 @default.
- W2189173652 cites W2139575177 @default.
- W2189173652 cites W2141351251 @default.
- W2189173652 cites W2145207381 @default.
- W2189173652 cites W2145817021 @default.
- W2189173652 cites W2146384070 @default.
- W2189173652 cites W2160239222 @default.
- W2189173652 cites W2160838897 @default.
- W2189173652 cites W2162679852 @default.
- W2189173652 cites W2163964271 @default.
- W2189173652 cites W2167168278 @default.
- W2189173652 cites W2395915186 @default.
- W2189173652 cites W2614847871 @default.
- W2189173652 doi "https://doi.org/10.1007/978-1-4614-8794-4_7" @default.
- W2189173652 hasPublicationYear "2013" @default.
- W2189173652 type Work @default.
- W2189173652 sameAs 2189173652 @default.
- W2189173652 citedByCount "0" @default.
- W2189173652 crossrefType "book-chapter" @default.
- W2189173652 hasAuthorship W2189173652A5031790890 @default.
- W2189173652 hasAuthorship W2189173652A5042126168 @default.
- W2189173652 hasConcept C1491633281 @default.
- W2189173652 hasConcept C169760540 @default.
- W2189173652 hasConcept C189014844 @default.
- W2189173652 hasConcept C203014093 @default.
- W2189173652 hasConcept C2775941552 @default.
- W2189173652 hasConcept C2776914184 @default.
- W2189173652 hasConcept C2777542381 @default.
- W2189173652 hasConcept C2777670902 @default.
- W2189173652 hasConcept C2779830541 @default.
- W2189173652 hasConcept C2780884517 @default.
- W2189173652 hasConcept C529278444 @default.
- W2189173652 hasConcept C54355233 @default.
- W2189173652 hasConcept C60644358 @default.
- W2189173652 hasConcept C81885089 @default.
- W2189173652 hasConcept C86803240 @default.
- W2189173652 hasConceptScore W2189173652C1491633281 @default.
- W2189173652 hasConceptScore W2189173652C169760540 @default.
- W2189173652 hasConceptScore W2189173652C189014844 @default.
- W2189173652 hasConceptScore W2189173652C203014093 @default.
- W2189173652 hasConceptScore W2189173652C2775941552 @default.
- W2189173652 hasConceptScore W2189173652C2776914184 @default.
- W2189173652 hasConceptScore W2189173652C2777542381 @default.
- W2189173652 hasConceptScore W2189173652C2777670902 @default.
- W2189173652 hasConceptScore W2189173652C2779830541 @default.
- W2189173652 hasConceptScore W2189173652C2780884517 @default.
- W2189173652 hasConceptScore W2189173652C529278444 @default.
- W2189173652 hasConceptScore W2189173652C54355233 @default.