Matches in SemOpenAlex for { <https://semopenalex.org/work/W2189218495> ?p ?o ?g. }
- W2189218495 endingPage "361" @default.
- W2189218495 startingPage "349" @default.
- W2189218495 abstract "The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM + plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM + plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles." @default.
- W2189218495 created "2016-06-24" @default.
- W2189218495 creator A5004601434 @default.
- W2189218495 creator A5007407541 @default.
- W2189218495 creator A5021848943 @default.
- W2189218495 creator A5027393058 @default.
- W2189218495 creator A5042369134 @default.
- W2189218495 creator A5044404097 @default.
- W2189218495 creator A5046492044 @default.
- W2189218495 creator A5061802421 @default.
- W2189218495 creator A5075252814 @default.
- W2189218495 creator A5087099636 @default.
- W2189218495 creator A5091311995 @default.
- W2189218495 date "2010-01-01" @default.
- W2189218495 modified "2023-09-22" @default.
- W2189218495 title "Expression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles" @default.
- W2189218495 cites W1963625504 @default.
- W2189218495 cites W1965803302 @default.
- W2189218495 cites W1983076004 @default.
- W2189218495 cites W1983782477 @default.
- W2189218495 cites W1996230138 @default.
- W2189218495 cites W1999150833 @default.
- W2189218495 cites W2005795262 @default.
- W2189218495 cites W2005958031 @default.
- W2189218495 cites W2012158660 @default.
- W2189218495 cites W2012362518 @default.
- W2189218495 cites W2015527564 @default.
- W2189218495 cites W2018853148 @default.
- W2189218495 cites W2021534970 @default.
- W2189218495 cites W2022632758 @default.
- W2189218495 cites W2024481165 @default.
- W2189218495 cites W2024497388 @default.
- W2189218495 cites W2028568478 @default.
- W2189218495 cites W2035414103 @default.
- W2189218495 cites W2036246831 @default.
- W2189218495 cites W2041314319 @default.
- W2189218495 cites W2049286581 @default.
- W2189218495 cites W2055634649 @default.
- W2189218495 cites W2056043788 @default.
- W2189218495 cites W2057482820 @default.
- W2189218495 cites W2064298474 @default.
- W2189218495 cites W2067874447 @default.
- W2189218495 cites W2069805450 @default.
- W2189218495 cites W2070466664 @default.
- W2189218495 cites W2074585597 @default.
- W2189218495 cites W2078307923 @default.
- W2189218495 cites W2078486422 @default.
- W2189218495 cites W2080496204 @default.
- W2189218495 cites W2082874604 @default.
- W2189218495 cites W2084843239 @default.
- W2189218495 cites W2089202971 @default.
- W2189218495 cites W2100480273 @default.
- W2189218495 cites W2105393647 @default.
- W2189218495 cites W2109228066 @default.
- W2189218495 cites W2138965475 @default.
- W2189218495 cites W2140786213 @default.
- W2189218495 cites W2150462205 @default.
- W2189218495 cites W2158716468 @default.
- W2189218495 cites W2159411410 @default.
- W2189218495 cites W2159940897 @default.
- W2189218495 cites W2160997583 @default.
- W2189218495 cites W2161283874 @default.
- W2189218495 cites W2165917193 @default.
- W2189218495 hasPublicationYear "2010" @default.
- W2189218495 type Work @default.
- W2189218495 sameAs 2189218495 @default.
- W2189218495 citedByCount "2" @default.
- W2189218495 countsByYear W21892184952015 @default.
- W2189218495 crossrefType "journal-article" @default.
- W2189218495 hasAuthorship W2189218495A5004601434 @default.
- W2189218495 hasAuthorship W2189218495A5007407541 @default.
- W2189218495 hasAuthorship W2189218495A5021848943 @default.
- W2189218495 hasAuthorship W2189218495A5027393058 @default.
- W2189218495 hasAuthorship W2189218495A5042369134 @default.
- W2189218495 hasAuthorship W2189218495A5044404097 @default.
- W2189218495 hasAuthorship W2189218495A5046492044 @default.
- W2189218495 hasAuthorship W2189218495A5061802421 @default.
- W2189218495 hasAuthorship W2189218495A5075252814 @default.
- W2189218495 hasAuthorship W2189218495A5087099636 @default.
- W2189218495 hasAuthorship W2189218495A5091311995 @default.
- W2189218495 hasConcept C102570824 @default.
- W2189218495 hasConcept C104317684 @default.
- W2189218495 hasConcept C105580179 @default.
- W2189218495 hasConcept C126322002 @default.
- W2189218495 hasConcept C134018914 @default.
- W2189218495 hasConcept C142773365 @default.
- W2189218495 hasConcept C16685009 @default.
- W2189218495 hasConcept C170493617 @default.
- W2189218495 hasConcept C175805485 @default.
- W2189218495 hasConcept C187785154 @default.
- W2189218495 hasConcept C196843134 @default.
- W2189218495 hasConcept C204232928 @default.
- W2189218495 hasConcept C2775960820 @default.
- W2189218495 hasConcept C2776188440 @default.
- W2189218495 hasConcept C2776690073 @default.
- W2189218495 hasConcept C2778324911 @default.
- W2189218495 hasConcept C2780536345 @default.
- W2189218495 hasConcept C2780689927 @default.