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• W2189269134 abstract "establish PCR methodology in the routine laboratory works. Th e total of  examined persons were divided in two groups: )  persons seropositive for HIV; and )  healthy persons - randomly selected blood donors that made the case control group. Th e subjects age was between  and  years (average ,). ELFA test for combined detection of HIV p antigen and anti HIV-+ IgG and ELISA test for detec- tion of antibodies against HIV- and HIV-, were performed for each examined person. RNA from the whole blood was extracted using a commercial kit based on salt precipitation. Detection of HIV RNA was performed using RT-PCR kit. Following nested PCR, the product was separated by electrophoresis in ,  agarose gel. Th e result was scored positive if the band of bp was visible regardless of intensity. Measures of precaution were taken during all the steps of the work and HIV infected materials were disposed of accordingly. In the group of blood donors ELFA, ELISA and RT-PCR were negative. Assuming that prevalence of HIV infection is zero, the clinical specifi city of RT-PCR is  . Th e analytical specifi city of RT-PCR method was tested against Hepatitis C and B, Human Papiloma Virus, Cytomegalovirus, Herpes Simplex Virus, Rubella Virus, Mycobacterium tuberculosis, Chlamydia trachomatis. None of these templates yielded am- plicon. In the group of  seropositive persons,  samples were analyzed. HIV RNA was detected in  samples. ELISA and ELFA test were positive in all samples. Diff erent aliquots of the samples were tested independently and showed the same results. After diff erent periods of storing the RNA samples at -oC, RT-PCR reaction was identical to the one performed initially. Th e obtained amplicons were maintained frozen at -oC for a week and the subsequently performed electrophoresis was identical to the previous one. Th e reaction is fast, simple for manipulation; with low detection level of  IU/ml. RT-PCR needs a small amount of RNA, as well as a small volume of sample. HIV RNA was detected in diff erent periods of time with diff erent clinical presentations in patients, with or without antiretroviral therapy. RT-PCR method gives the opportunity for reliable determination of HIV- RNA with border of detection of  IU/ml. Th e test is reproducible and has high analytical and clinical specifi city." @default.
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• W2189269134 date "2008-01-01" @default.
• W2189269134 modified "2023-09-27" @default.
• W2189269134 title "RT-PCR DETECTION OF HIV IN REPUBLIC OF" @default.
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