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- W2189275332 abstract "Resistance (R) genes containing nucleotide binding site (NBS) and leucine rich repeats (LRR) are the most prevalent types of resistance (R) genes in plants. The objective of this study was to isolate, identify and analyze resistance genes from disease (rust) and resistant wheat lines by PCR based strategy. Fifteen degenerate primers were designed from the conserved kinase-la and hydrophobic domains of known NBS-LRR type R-genes and from EST data bases. Four advanced resistant lines and one susceptible wheat line was selected from the trap nursery. Out of hundred primer combinations only seventy five primer combinations showed amplification. Twenty two primer combination showed differential banding pattern which were not present in highly susceptible Morocco, were cloned in TA based cloning vector and got them sequenced. Sizes of sequenced nucleotides were between 500bp to 1500bp. The cloned fragments showed their DNA sequence similarity to known resistance (R) genes of NBS-LRR family. These results indicate that identified genes are the valuable source to use as disease resistance genes or to screen wheat resistant germplasm against different types of rusts." @default.
- W2189275332 created "2016-06-24" @default.
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- W2189275332 date "2014-01-01" @default.
- W2189275332 modified "2023-09-27" @default.
- W2189275332 title "Cloning and analysis of NBS-LRR super family of resistance (R) genes in wheat (Triticum aestivum L.)" @default.
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