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- W2189277356 abstract "Now, the comet assay is used widely to detect chemical genotoxicity both in vitro and in vivo. There may be many important factors affecting the outcomes of in vitro comet assay, for example, exposure and sampling periods, used cells, S9 components, and electrophoresis condition. We have been questioning better experimental design to give good outcome of this assay. Followings are our tentative conclusions: (1) long sampling period should not be employed; (2) long exposure period should not be employed for chemicals that are unstable in culture media; (3) any eukaryotic cells can be used in this assay; (4) S9 components same as those used in the Ames test can be used to give good outcomes in this assay; and (5) although different kinds of DNA lesion can be detected at different pH condition during electrophoresis, alkaline (pH >13) condition is suitable for the comet assay as a genotoxicity test." @default.
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- W2189277356 date "2008-01-01" @default.
- W2189277356 modified "2023-09-24" @default.
- W2189277356 title "What is better experimental design for in vitro comet assay to detect chemical genotoxicity" @default.
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