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- W2189281750 abstract "The aim of the present paper was the comparative assessment of two ELISA tests on serum and milk samples and agar gel immunodiffusion test (AGID) regarding ability to detect enzootic bovine leukosis (EBL) in Timis County, according to farm type, age, breed and stage of lactation, and how these parameters affect the humoral immune response of positive cattle. Serum samples were analyzed using blocking ELISA and AGID, and the milk samples by indirect ELISA, calculating quality parameters of applied tests. The obtained results showed that AGID detected 43 positive cattle and the two ELISAs 51 positive animals. Performances of AGID were influenced by farm type, age and serum antibody level. It was established that cattle aged 3-6 years were most prone to develop EBL. The level of serum antibodies has been influenced by farm type, age and lactation phase, and milk antibodies level strictly by the phase of lactation; the lowest values of milk antibodies were registered between 2 nd and 8 th month of lactation. Consumption of insufficiently heat-treated milk from BLV positive cattle in 2-8 months of lactation is associated with increased risk of infection in humans." @default.
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- W2189281750 date "2014-01-01" @default.
- W2189281750 modified "2023-10-18" @default.
- W2189281750 title "Performance assessment of three tests applied in enzootic bovine leukosis diagnosis" @default.
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