Matches in SemOpenAlex for { <https://semopenalex.org/work/W2189302124> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2189302124 abstract "Summary Actinomycete strains isolated from marine sediment samples collected at the Bay of Bengal coast of Puddcherry, India was screened for antiproliferative activity on three cancer cell lines by trypan blue staining and MTT assay. The potential isolate having antiproliferative activity was cultured on ISP -Veg media supplemented with sea water, pH 7.2, and the inoculated broth was maintained at 28° C for 7 days. The media and cultural conditions for maximal growth have been optimized under shakeflask conditions by measuring the dry weight of the mycelium. The maximal growth was attained with the use of optimized production medium, pH of 7.2 and incubation temperature of 27°C. Based on molecular taxonomic and phylogenetic characterization, the strain was identified as Rhodococcus and designated as Rhodococcus sp. VITSNK6. The 16 S rDNA nucleotide sequence from the 16 S rRNA partial gene sequence of the isolate was deposited in the GenBank under the sequence of the accession number FJ 973467. The ethyl acetate extract prepared from the supernatant of the culture broth was used for testing the antiprolifertive activity against HeLa (human cervical carcinoma), MDAMB 231 (human breast adenocarcinoma), HepG2 (hepatocellular carcinoma) cells, HEK (Human embryonic kidney) and Vero (green monkey kidney) cell lines. The susceptibility of cancer cell lines to the extract was in the order of Hep G2> MDAMB 2 31>HeLa with the IC 50 values 22 Ag/ml, 35 Ag/ml and 45 Ag/ml respectively. The extr act treated cancer cells showed apoptosis mediated cell death and the viability of cancer cells was inversely proportional to the concentration of the extract used. The normal cell lines were less susceptible to inhibition than cancer cells. The results of this study showed that the secondary metabolite produced by the isolate has marked antiproliferative activity against cancer cells." @default.
- W2189302124 created "2016-06-24" @default.
- W2189302124 creator A5048245663 @default.
- W2189302124 date "2009-01-01" @default.
- W2189302124 modified "2023-09-27" @default.
- W2189302124 title "ATIPROLIFERATIVE ACTIVITY OF RHODOCOCCUS VITSK6 ISOLATED FROM MARIE SEDIMET SAMPLES COLLECTED AT THE PUDUCHERRY COAST, BAY OF BEGAL, IDIA" @default.
- W2189302124 cites W1515477239 @default.
- W2189302124 cites W1933320224 @default.
- W2189302124 cites W1970935314 @default.
- W2189302124 cites W1972119811 @default.
- W2189302124 cites W1977188155 @default.
- W2189302124 cites W1979668881 @default.
- W2189302124 cites W2009156991 @default.
- W2189302124 cites W2017773215 @default.
- W2189302124 cites W2024173873 @default.
- W2189302124 cites W2029697661 @default.
- W2189302124 cites W2045119872 @default.
- W2189302124 cites W2060522791 @default.
- W2189302124 cites W2097706568 @default.
- W2189302124 cites W2119243174 @default.
- W2189302124 cites W2132700081 @default.
- W2189302124 cites W2171513236 @default.
- W2189302124 cites W2285949578 @default.
- W2189302124 hasPublicationYear "2009" @default.
- W2189302124 type Work @default.
- W2189302124 sameAs 2189302124 @default.
- W2189302124 citedByCount "0" @default.
- W2189302124 crossrefType "journal-article" @default.
- W2189302124 hasAuthorship W2189302124A5048245663 @default.
- W2189302124 hasConcept C153911025 @default.
- W2189302124 hasConcept C190283241 @default.
- W2189302124 hasConcept C2777366897 @default.
- W2189302124 hasConcept C2780070996 @default.
- W2189302124 hasConcept C54355233 @default.
- W2189302124 hasConcept C55493867 @default.
- W2189302124 hasConcept C81885089 @default.
- W2189302124 hasConcept C86803240 @default.
- W2189302124 hasConcept C89423630 @default.
- W2189302124 hasConceptScore W2189302124C153911025 @default.
- W2189302124 hasConceptScore W2189302124C190283241 @default.
- W2189302124 hasConceptScore W2189302124C2777366897 @default.
- W2189302124 hasConceptScore W2189302124C2780070996 @default.
- W2189302124 hasConceptScore W2189302124C54355233 @default.
- W2189302124 hasConceptScore W2189302124C55493867 @default.
- W2189302124 hasConceptScore W2189302124C81885089 @default.
- W2189302124 hasConceptScore W2189302124C86803240 @default.
- W2189302124 hasConceptScore W2189302124C89423630 @default.
- W2189302124 hasLocation W21893021241 @default.
- W2189302124 hasOpenAccess W2189302124 @default.
- W2189302124 hasPrimaryLocation W21893021241 @default.
- W2189302124 hasRelatedWork W2124943439 @default.
- W2189302124 hasRelatedWork W2126485749 @default.
- W2189302124 hasRelatedWork W2159076071 @default.
- W2189302124 hasRelatedWork W2181932498 @default.
- W2189302124 hasRelatedWork W2359609648 @default.
- W2189302124 hasRelatedWork W2362945632 @default.
- W2189302124 hasRelatedWork W2376358477 @default.
- W2189302124 hasRelatedWork W2386443017 @default.
- W2189302124 hasRelatedWork W2746149455 @default.
- W2189302124 hasRelatedWork W2768165046 @default.
- W2189302124 hasRelatedWork W2807926600 @default.
- W2189302124 hasRelatedWork W2887323494 @default.
- W2189302124 hasRelatedWork W2936193733 @default.
- W2189302124 hasRelatedWork W2936688143 @default.
- W2189302124 hasRelatedWork W2948309422 @default.
- W2189302124 hasRelatedWork W3006323469 @default.
- W2189302124 hasRelatedWork W3202390179 @default.
- W2189302124 hasRelatedWork W3211710979 @default.
- W2189302124 hasRelatedWork W2184561934 @default.
- W2189302124 hasRelatedWork W2188932443 @default.
- W2189302124 isParatext "false" @default.
- W2189302124 isRetracted "false" @default.
- W2189302124 magId "2189302124" @default.
- W2189302124 workType "article" @default.