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- W2189307985 abstract "TheTiplasmids ofAgrobacterium tume- faciens areconjugal elements whosetransfer isstrongly re- pressed. Transfer isinduced bytheconjugal opines, agroup of unique carbon compounds synthesized incrowngail tumors. Theopines also induce Tiplasmid-encoded genes required by thebacteria for' opine catabolism. We havecloned andse- quenced agenefromtheTiplasmid pTiC58, whoseproduct mediates theopine-dependent regulation ofconjugal transfer andcatabolism oftheconjugal opines, agrocinopines AandB. Thegene, accR, isclosely linked totheagrocinopine catabolic locus. A spontaneous mutant Tiplasmid, pTiC58Trac, which constitutively expresses conjugal transfer andopine catabo- lism, wascomplemented intrans byaclone ofwild-type accR. Comparative sequence analysis identified a5-base-pair dele- tionclose tothe5'endofthemutantaccRallele from pTiC58Trac. Analysis oflacZ fusions inconjugal transfer and opine catabolic structural genes demonstrated that theaccR- encoded function isa transcriptional repressor. accRcan encode a28-kDa protein. Thisprotein isrelated toaclass of repressor proteins that includes LacR, GutR, DeoR,FucR, and GlpRthatregulate sugar catabolic systems inseveral bacterial genera." @default.
- W2189307985 created "2016-06-24" @default.
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- W2189307985 date "1992-01-01" @default.
- W2189307985 modified "2023-09-26" @default.
- W2189307985 title "Opinecatabolism andconjugal transfer Qfthenopaline Tiplasmid pTiC58arecoordinately regulated byasingle repressor" @default.
- W2189307985 hasPublicationYear "1992" @default.
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