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- W2189409713 abstract "A DNA-membrane complex extracted fromBacillus subtilis was studied further as a modelsystemfor initiation ofbacterial DNA replication invitro. Ofthree subcomplexes purified fromthecrudecomplex bya combination ofCsClandsucrosegradient centrifugation, thesynthetic capability ofonlyone was inhibited significantly bystreptovaricin, aknowninhibitor ofRNA primer formation. A selective enrichment inthelevel ofthis subcomplex was obtained bymanipulating athymine-requiring mutant.Thesynthetic capabilities ofan enriched andnonenriched DNA-membranecomplex were comparedinthepresence and absenceof streptovaricin. Although therateandextent ofDNA synthesis perunitofprotein were approximately thesame intheabsence oftheantibiotic, there was amuchgreater inhibition ofsynthesis shownbytheenriched complex inthepresenceofstreptovaricin. Although theamountofDNA present intheputative initiation subcomplex was less than0.3to0.4%ofthetotal DNA presentinthecrudecomplex, suchDNA, exceptfora few quantitative differences, was still representative ofgenomic DNA.Newlysynthesized DNA hybridized to specific origin- andnon-origin-derived restriction fragments oftheB.subtilis genome. However, whenan elongation inhibitor (ddCTP) was added, hybridization ofsuchDNA toalmost allofthenonorigin fragments disappeared or was reduced drastically, whereas origin region hybridization patterns remained strong. The highest level ofhybridization intheorigin region occurred witha BamHI(B7)restriction fragment of5.6 kilobases thathasbeenimplicated byothers asone site ofinitiation invivo(N.Ogasawara, M.Seiki, andH. Yoshikawa, Nature (London) 281:702-704, 1979; S.J.Seror-Laurent andG.Henckes, Proc.Natl. Acad.Sci. USA82:3586-3590, 1985)." @default.
- W2189409713 created "2016-06-24" @default.
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- W2189409713 date "1987-01-01" @default.
- W2189409713 modified "2023-09-27" @default.
- W2189409713 title "DNA Replication bya DNA-MembraneComplexExtracted from Bacillus subtilis: Site ofInitiation InVitro and Initiation Potential ofSubcomplexes" @default.
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