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• W2189542719 abstract "Translational diffusion coefficient D of a protein in a solution depends both on temperature (T) and the protein’s volume fraction (Φ). At infinitely dilute solutions the translational diffusion coefficient Do(T) can be calculated from Stokes-Einstein formula, if the hydrodynamic radius of dissolved protein is known. For hydrated hen egg-white lysozyme this quantity is equal to 1.95 nm, and it gives Do(T) in the range from 6.89×10 -11 m 2 /s (at 5 o C) to 24.4×10 -11 m 2 /s (at 55 o C). For higher concentrations D can obtain from the relation: D(T,Φ) = Do(T)ηo(T)/η(T,Φ), where ηo(T) and η(T,Φ) are viscosities of water and solution, respectively, at temperature T. To obtain D(T,Φ) the viscosity of hen egg-white lysozyme aqueous solutions has been measured at temperatures ranging from 5 o C to 55 o C and for volume fraction from 0.023 to 0.315. The dependence of D(T,Φ) – obtained indirectly on the basis of viscosity measurements - on Φ (at fixed temperature) has been analyzed on the basis of a stretched exponential function: D(T,Φ) = Do(T)exp(βΦ ν ), where β and ν are scaling parameters. Both parameters decreases with increasing temperature: β from 13.2 (5 o C) to 7.83 o C) and ν from 1.4 (5 o C) to 1.26 (55 o C). The dependence of D(T,Φ) on T (at fixed volume fraction), in turn, has been analyzed on the basis of the Vogel-Tammann-Fulcher’s equation." @default.
• W2189542719 created "2016-06-24" @default.
• W2189542719 creator A5001216029 @default.
• W2189542719 date "2009-01-01" @default.
• W2189542719 modified "2023-09-27" @default.
• W2189542719 title "c,T - DEPENDENCE OF THE TRANSLATIONAL DIFFUSION COEFFICIENT FOR HEN EGG-WHITE LYSOZYME IN AQUEOUS SOLUTIONS OBTAINED FROM VISCOSITY MEASUREMENTS AND GENERALIZED STOKES-EINSTEIN RELATION." @default.
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