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• W2189546817 abstract "The prevalence and mechanisms of antibiotic resistance of He licobacter pylori have not yet been investigated in Uruguay. The objective of this study was to assess the susceptibility of H. pylori to the most frequently used antibiotics and to determine the mech anism of resistance to clarithromycin. Seventy-nine isolates were obtained from gastric biopsies of 50 adult patients during two pe riods, 2001 and 2006. The former group enrolled the general population (GP), the latter group Afro-descendant (AD) subjects. The minimum inhibitory concentrations of clarithromycin, amoxicillin, tetracycline, metronidazole, and levofloxacin were deter mined using the E-test technique. Amplification was achieved through PCR and nucleic acid sequencing to detect mutations in the site of action of clarithromycin in the rRNA gene 23S. No amoxicillin or tetracycline-resistant strains were found. Clar ithromycin resistance was found in 12% of the patients overall: 19.4% resistance in AD patients and no resistance in the GP group. This difference was statistically significant. The highest re sistance was seen with metronidazole (36%), present in similar proportions in the two groups: 36.8% (GP) and 35.5% (AD). One GP patient and one AD patient had levofloxacin-resistant strains. Sequencing analysis of gene 23S rRNA showed that only muta tion in position 2143 was presented in all clarithromycin-resistant strains." @default.
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• W2189546817 date "2009-01-01" @default.
• W2189546817 modified "2023-10-08" @default.
• W2189546817 title "Antimicrobialsusceptibilityof Helicobacter pyloriand mechanismsofclarithromycinresistanceinstrainsisolatedfrom patientsinUruguay" @default.
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