FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2190468334> ?p ?o ?g. }

• W2190468334 endingPage "4388" @default.
• W2190468334 startingPage "4383" @default.
• W2190468334 abstract "In the specialized ribosome system, a distinct pool of mutated ribosomes is dedicated to the translation of one particular mRNA species. This was accomplished by altering the Shine-Dalgarno sequence on the mRNA and its complementary anti-Shine-Dalgarno sequence on the plasmid-borne 16S rRNA gene. Here, using the specialized ribosome system, we were able to introduce mutations in key regions of the 16S rRNA and could study their effect on translation in vivo. The C1400 region has been implicated to play a role in the actual mRNA decoding process. Several ribosomal mutations were introduced in this region. We showed that substitution of the evolutionary highly conserved C1400 residue by a G- or an A-residue inhibits ribosomal activity by 80% and 50% respectively, whereas, a C to a U change at this conserved position does not affect overall ribosomal activity. The adjacent stem structure (1410-1490) was also examined. Disruption of the stem by replacing either one of the arms of this stem, with a different sequence, inhibits ribosomal activity by approximately 80%. A small but significant restoration of translation could be achieved by recreating a complementary stem with a different sequence. We found that full reversion of activity could be obtained when such mutated ribosomes were made spectinomycin resistant by introducing a C to A substitution at position 1192 which is located far away in the secondary structure map of the 16S rRNA molecule. Based on these results we conclude that some, but not all, of the nucleotides in the conserved C1400 region play a key role in translation.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2190468334 created "2016-06-24" @default.
• W2190468334 creator A5015375220 @default.
• W2190468334 creator A5030135003 @default.
• W2190468334 creator A5034134883 @default.
• W2190468334 date "1988-12-01" @default.
• W2190468334 modified "2023-09-27" @default.
• W2190468334 title "Mutagenesis at the mRNA decoding site in the 16S ribosomal RNA using the specialized ribosome system in Escherichia coli." @default.
• W2190468334 cites W1176358574 @default.
• W2190468334 cites W1547205596 @default.
• W2190468334 cites W1567282410 @default.
• W2190468334 cites W1597786818 @default.
• W2190468334 cites W1994416898 @default.
• W2190468334 cites W1999926095 @default.
• W2190468334 cites W2018429609 @default.
• W2190468334 cites W2024263653 @default.
• W2190468334 cites W2026358434 @default.
• W2190468334 cites W2048648042 @default.
• W2190468334 cites W2055627617 @default.
• W2190468334 cites W2064115229 @default.
• W2190468334 cites W2064560761 @default.
• W2190468334 cites W2065612681 @default.
• W2190468334 cites W2065666222 @default.
• W2190468334 cites W2081823929 @default.
• W2190468334 cites W2087064814 @default.
• W2190468334 cites W2121234754 @default.
• W2190468334 cites W2142393243 @default.
• W2190468334 cites W2157010941 @default.
• W2190468334 cites W2174266714 @default.
• W2190468334 doi "https://doi.org/10.1002/j.1460-2075.1988.tb03337.x" @default.
• W2190468334 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/455173" @default.
• W2190468334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2468489" @default.
• W2190468334 hasPublicationYear "1988" @default.
• W2190468334 type Work @default.
• W2190468334 sameAs 2190468334 @default.
• W2190468334 citedByCount "43" @default.
• W2190468334 countsByYear W21904683342017 @default.
• W2190468334 countsByYear W21904683342020 @default.
• W2190468334 countsByYear W21904683342021 @default.
• W2190468334 countsByYear W21904683342022 @default.
• W2190468334 countsByYear W21904683342023 @default.
• W2190468334 crossrefType "journal-article" @default.
• W2190468334 hasAuthorship W2190468334A5015375220 @default.
• W2190468334 hasAuthorship W2190468334A5030135003 @default.
• W2190468334 hasAuthorship W2190468334A5034134883 @default.
• W2190468334 hasBestOaLocation W21904683342 @default.
• W2190468334 hasConcept C104317684 @default.
• W2190468334 hasConcept C105580179 @default.
• W2190468334 hasConcept C114847915 @default.
• W2190468334 hasConcept C129194359 @default.
• W2190468334 hasConcept C149364088 @default.
• W2190468334 hasConcept C153911025 @default.
• W2190468334 hasConcept C38062823 @default.
• W2190468334 hasConcept C52721335 @default.
• W2190468334 hasConcept C54355233 @default.
• W2190468334 hasConcept C55334214 @default.
• W2190468334 hasConcept C67705224 @default.
• W2190468334 hasConcept C67905577 @default.
• W2190468334 hasConcept C86803240 @default.
• W2190468334 hasConcept C88478588 @default.
• W2190468334 hasConcept C91779695 @default.
• W2190468334 hasConcept C98098607 @default.
• W2190468334 hasConceptScore W2190468334C104317684 @default.
• W2190468334 hasConceptScore W2190468334C105580179 @default.
• W2190468334 hasConceptScore W2190468334C114847915 @default.
• W2190468334 hasConceptScore W2190468334C129194359 @default.
• W2190468334 hasConceptScore W2190468334C149364088 @default.
• W2190468334 hasConceptScore W2190468334C153911025 @default.
• W2190468334 hasConceptScore W2190468334C38062823 @default.
• W2190468334 hasConceptScore W2190468334C52721335 @default.
• W2190468334 hasConceptScore W2190468334C54355233 @default.
• W2190468334 hasConceptScore W2190468334C55334214 @default.
• W2190468334 hasConceptScore W2190468334C67705224 @default.
• W2190468334 hasConceptScore W2190468334C67905577 @default.
• W2190468334 hasConceptScore W2190468334C86803240 @default.
• W2190468334 hasConceptScore W2190468334C88478588 @default.
• W2190468334 hasConceptScore W2190468334C91779695 @default.
• W2190468334 hasConceptScore W2190468334C98098607 @default.
• W2190468334 hasIssue "13" @default.
• W2190468334 hasLocation W21904683341 @default.
• W2190468334 hasLocation W21904683342 @default.
• W2190468334 hasLocation W21904683343 @default.
• W2190468334 hasOpenAccess W2190468334 @default.
• W2190468334 hasPrimaryLocation W21904683341 @default.
• W2190468334 hasRelatedWork W198985796 @default.
• W2190468334 hasRelatedWork W2001504992 @default.
• W2190468334 hasRelatedWork W2046651133 @default.
• W2190468334 hasRelatedWork W2047745452 @default.
• W2190468334 hasRelatedWork W2190468334 @default.
• W2190468334 hasRelatedWork W2752407965 @default.
• W2190468334 hasRelatedWork W2907618003 @default.
• W2190468334 hasRelatedWork W4220902340 @default.
• W2190468334 hasRelatedWork W5704356 @default.
• W2190468334 hasRelatedWork W941015034 @default.
• W2190468334 hasVolume "7" @default.
• W2190468334 isParatext "false" @default.
• W2190468334 isRetracted "false" @default.
• W2190468334 magId "2190468334" @default.

• next