FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2191290165> ?p ?o ?g. }

• W2191290165 abstract "A novel enzyme-linked receptor assay (ELRA) based on β2-adrenergic receptor (β2-AR) has been developed for rapid and high-throughput detection of β-adrenergic agonists (β-agonists) in urine. Human embryonic kidney cells (HEK293) were introduced as the expression system to enhance the functionality of the recombinant β2-AR, and the attempt to detect β-agonists in swine urine using such approaches was accomplished unprecedentedly. In this article, a recombinant porcine β2-AR was produced in the inner membrane of HEK293 cells and purified from crude membrane protein by nickel-nitrilotriacetic acid affinity chromatography. After activity identification, the recombinant receptor was used in the development of direct competitive ELRA. Several parameters such as blocking buffer and blocking process were optimized and the performance of the system was determined. The IC50 concentrations of clenbuterol, salbutamol, and ractopamine were 34, 53 and 63 μg/L, and the average recovery rates were 68.2%, 60.3% and 65.5%, respectively. ELRA based on β2-AR shows a series of advantages such as safety, easy operation, and high efficiency, making it promising for the rapid screening of β-agonists in animal urine." @default.
• W2191290165 created "2016-06-24" @default.
• W2191290165 creator A5015611625 @default.
• W2191290165 creator A5032764121 @default.
• W2191290165 creator A5037570142 @default.
• W2191290165 creator A5037677450 @default.
• W2191290165 creator A5045963998 @default.
• W2191290165 creator A5046214153 @default.
• W2191290165 creator A5061082958 @default.
• W2191290165 date "2015-09-30" @default.
• W2191290165 modified "2023-09-25" @default.
• W2191290165 title "Multiresidue Method for Analysis of β Agonists in Swine Urine by Enzyme Linked Receptor Assay Based on β2 Adrenergic Receptor Expressed in HEK293 Cells" @default.
• W2191290165 cites W1600615773 @default.
• W2191290165 cites W1825353903 @default.
• W2191290165 cites W1973739813 @default.
• W2191290165 cites W1975637301 @default.
• W2191290165 cites W1978495788 @default.
• W2191290165 cites W1984767654 @default.
• W2191290165 cites W1985571627 @default.
• W2191290165 cites W1988634322 @default.
• W2191290165 cites W2000333563 @default.
• W2191290165 cites W2019277873 @default.
• W2191290165 cites W2020130922 @default.
• W2191290165 cites W2026062301 @default.
• W2191290165 cites W2027150656 @default.
• W2191290165 cites W2027222713 @default.
• W2191290165 cites W2028496426 @default.
• W2191290165 cites W2031024782 @default.
• W2191290165 cites W2034516270 @default.
• W2191290165 cites W2035828382 @default.
• W2191290165 cites W2037839687 @default.
• W2191290165 cites W2043405071 @default.
• W2191290165 cites W2048528605 @default.
• W2191290165 cites W2066104325 @default.
• W2191290165 cites W2075214402 @default.
• W2191290165 cites W2080680089 @default.
• W2191290165 cites W2083722349 @default.
• W2191290165 cites W2091030689 @default.
• W2191290165 cites W2110917535 @default.
• W2191290165 cites W2133377634 @default.
• W2191290165 cites W2153107242 @default.
• W2191290165 cites W2159718916 @default.
• W2191290165 cites W2161527536 @default.
• W2191290165 cites W2315575171 @default.
• W2191290165 cites W2399790174 @default.
• W2191290165 cites W851175451 @default.
• W2191290165 doi "https://doi.org/10.1371/journal.pone.0139176" @default.
• W2191290165 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4589316" @default.
• W2191290165 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26422475" @default.
• W2191290165 hasPublicationYear "2015" @default.
• W2191290165 type Work @default.
• W2191290165 sameAs 2191290165 @default.
• W2191290165 citedByCount "6" @default.
• W2191290165 countsByYear W21912901652015 @default.
• W2191290165 countsByYear W21912901652017 @default.
• W2191290165 countsByYear W21912901652018 @default.
• W2191290165 countsByYear W21912901652019 @default.
• W2191290165 countsByYear W21912901652020 @default.
• W2191290165 crossrefType "journal-article" @default.
• W2191290165 hasAuthorship W2191290165A5015611625 @default.
• W2191290165 hasAuthorship W2191290165A5032764121 @default.
• W2191290165 hasAuthorship W2191290165A5037570142 @default.
• W2191290165 hasAuthorship W2191290165A5037677450 @default.
• W2191290165 hasAuthorship W2191290165A5045963998 @default.
• W2191290165 hasAuthorship W2191290165A5046214153 @default.
• W2191290165 hasAuthorship W2191290165A5061082958 @default.
• W2191290165 hasBestOaLocation W21912901651 @default.
• W2191290165 hasConcept C104317684 @default.
• W2191290165 hasConcept C153911025 @default.
• W2191290165 hasConcept C170493617 @default.
• W2191290165 hasConcept C174510640 @default.
• W2191290165 hasConcept C181199279 @default.
• W2191290165 hasConcept C185592680 @default.
• W2191290165 hasConcept C202751555 @default.
• W2191290165 hasConcept C2777752497 @default.
• W2191290165 hasConcept C2778938600 @default.
• W2191290165 hasConcept C2780458105 @default.
• W2191290165 hasConcept C40767141 @default.
• W2191290165 hasConcept C43617362 @default.
• W2191290165 hasConcept C55493867 @default.
• W2191290165 hasConcept C86803240 @default.
• W2191290165 hasConcept C98274493 @default.
• W2191290165 hasConceptScore W2191290165C104317684 @default.
• W2191290165 hasConceptScore W2191290165C153911025 @default.
• W2191290165 hasConceptScore W2191290165C170493617 @default.
• W2191290165 hasConceptScore W2191290165C174510640 @default.
• W2191290165 hasConceptScore W2191290165C181199279 @default.
• W2191290165 hasConceptScore W2191290165C185592680 @default.
• W2191290165 hasConceptScore W2191290165C202751555 @default.
• W2191290165 hasConceptScore W2191290165C2777752497 @default.
• W2191290165 hasConceptScore W2191290165C2778938600 @default.
• W2191290165 hasConceptScore W2191290165C2780458105 @default.
• W2191290165 hasConceptScore W2191290165C40767141 @default.
• W2191290165 hasConceptScore W2191290165C43617362 @default.
• W2191290165 hasConceptScore W2191290165C55493867 @default.
• W2191290165 hasConceptScore W2191290165C86803240 @default.
• W2191290165 hasConceptScore W2191290165C98274493 @default.
• W2191290165 hasLocation W21912901651 @default.
• W2191290165 hasLocation W21912901652 @default.
• W2191290165 hasLocation W21912901653 @default.

• next