FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2192971349> ?p ?o ?g. }

• W2192971349 endingPage "43" @default.
• W2192971349 startingPage "1432" @default.
• W2192971349 abstract "Although rat liver epithelial cell (RLEC) lines have been developed by a number of laboratories, the identity of the clonogenic nonparenchymal progenitors is unknown. To provide insight into the derivation of RLEC, we immunoisolated serosal liver mesothelial cells (LMC) and bile duct epithelial cells and attempted to propagate each epithelial cell population using culture conditions routinely employed to establish RLEC lines. Briefly, the selective reactivity of LMC with two bile duct cell surface markers, OC.2 and BD.2, was exploited to develop an immunocytochemical technique to isolate LMC. Livers were collagenase dissociated, the mesothelial capsule was peeled and digested with pronase to destroy contaminating hepatocytes, and rare biliary ductal epithelial cells were immunodepleted using OC.2. LMC were subsequently isolated by selective binding to magnetic beads adsorbed with BD.2 and cultured in supplemented Waymouths 752/1 media containing 10% fetal calf serum. Proliferating BD.2+ LMC rapidly formed epithelial-like monolayers that could be continuously subcultured after trypsinization. In contrast, attempts to establish cell lines from purified OC.2+ bile duct epithelial cells were unsuccessful. Results from reverse transcriptase polymerase chain reaction analysis confirmed that LMC expressed Wilms' tumor transcripts, a lineage marker for mesodermally-derived cells. In summary, our findings clearly demonstrate that LMC can be continuously propagated using culture conditions routinely employed to establish RLEC lines, an observation that supports the contention that some RLEC lines may be derived from LMC." @default.
• W2192971349 created "2016-06-24" @default.
• W2192971349 creator A5013233751 @default.
• W2192971349 creator A5020242220 @default.
• W2192971349 creator A5021784904 @default.
• W2192971349 creator A5060545410 @default.
• W2192971349 creator A5079473941 @default.
• W2192971349 creator A5088746067 @default.
• W2192971349 date "1994-12-01" @default.
• W2192971349 modified "2023-09-27" @default.
• W2192971349 title "Isolation, propagation, and characterization of rat liver serosal mesothelial cells." @default.
• W2192971349 cites W143089095 @default.
• W2192971349 cites W150633023 @default.
• W2192971349 cites W1506860172 @default.
• W2192971349 cites W1510596780 @default.
• W2192971349 cites W1516759786 @default.
• W2192971349 cites W1533476609 @default.
• W2192971349 cites W155790071 @default.
• W2192971349 cites W1558024245 @default.
• W2192971349 cites W1567101341 @default.
• W2192971349 cites W1593105968 @default.
• W2192971349 cites W1607965217 @default.
• W2192971349 cites W1616471700 @default.
• W2192971349 cites W1646941628 @default.
• W2192971349 cites W1661400113 @default.
• W2192971349 cites W1910452754 @default.
• W2192971349 cites W1911914287 @default.
• W2192971349 cites W1912522449 @default.
• W2192971349 cites W1966569385 @default.
• W2192971349 cites W1972487225 @default.
• W2192971349 cites W1983755974 @default.
• W2192971349 cites W1991501430 @default.
• W2192971349 cites W1992644378 @default.
• W2192971349 cites W1996109421 @default.
• W2192971349 cites W1997100869 @default.
• W2192971349 cites W2002779176 @default.
• W2192971349 cites W2011565183 @default.
• W2192971349 cites W2025237524 @default.
• W2192971349 cites W2026725936 @default.
• W2192971349 cites W2035160128 @default.
• W2192971349 cites W2037784945 @default.
• W2192971349 cites W2043957483 @default.
• W2192971349 cites W2044772898 @default.
• W2192971349 cites W2059122016 @default.
• W2192971349 cites W2060333964 @default.
• W2192971349 cites W2071180078 @default.
• W2192971349 cites W2072482644 @default.
• W2192971349 cites W2072891772 @default.
• W2192971349 cites W2075573504 @default.
• W2192971349 cites W2081631539 @default.
• W2192971349 cites W2084508917 @default.
• W2192971349 cites W2086658389 @default.
• W2192971349 cites W2097772140 @default.
• W2192971349 cites W2132584547 @default.
• W2192971349 cites W2141617559 @default.
• W2192971349 cites W2142133226 @default.
• W2192971349 cites W2152151858 @default.
• W2192971349 cites W2160818570 @default.
• W2192971349 cites W2170478111 @default.
• W2192971349 cites W2194803086 @default.
• W2192971349 cites W2331630900 @default.
• W2192971349 cites W2412264144 @default.
• W2192971349 cites W2576734941 @default.
• W2192971349 cites W65170271 @default.
• W2192971349 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1887510" @default.
• W2192971349 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7992846" @default.
• W2192971349 hasPublicationYear "1994" @default.
• W2192971349 type Work @default.
• W2192971349 sameAs 2192971349 @default.
• W2192971349 citedByCount "6" @default.
• W2192971349 countsByYear W21929713492016 @default.
• W2192971349 countsByYear W21929713492017 @default.
• W2192971349 crossrefType "journal-article" @default.
• W2192971349 hasAuthorship W2192971349A5013233751 @default.
• W2192971349 hasAuthorship W2192971349A5020242220 @default.
• W2192971349 hasAuthorship W2192971349A5021784904 @default.
• W2192971349 hasAuthorship W2192971349A5060545410 @default.
• W2192971349 hasAuthorship W2192971349A5079473941 @default.
• W2192971349 hasAuthorship W2192971349A5088746067 @default.
• W2192971349 hasConcept C117262875 @default.
• W2192971349 hasConcept C126322002 @default.
• W2192971349 hasConcept C142724271 @default.
• W2192971349 hasConcept C153911025 @default.
• W2192971349 hasConcept C181199279 @default.
• W2192971349 hasConcept C201750760 @default.
• W2192971349 hasConcept C2778394429 @default.
• W2192971349 hasConcept C2779777945 @default.
• W2192971349 hasConcept C2780340462 @default.
• W2192971349 hasConcept C28328180 @default.
• W2192971349 hasConcept C2908647359 @default.
• W2192971349 hasConcept C2909640154 @default.
• W2192971349 hasConcept C38240268 @default.
• W2192971349 hasConcept C529295009 @default.
• W2192971349 hasConcept C54355233 @default.
• W2192971349 hasConcept C55493867 @default.
• W2192971349 hasConcept C71924100 @default.
• W2192971349 hasConcept C81885089 @default.
• W2192971349 hasConcept C86803240 @default.

• next