Matches in SemOpenAlex for { <https://semopenalex.org/work/W2192991381> ?p ?o ?g. }
- W2192991381 endingPage "6440" @default.
- W2192991381 startingPage "6424" @default.
- W2192991381 abstract "Bacteriophage T5, a Siphovirus belonging to the order Caudovirales, has a flexible, three-fold symmetric tail, to which three L-shaped fibres are attached. These fibres recognize oligo-mannose units on the bacterial cell surface prior to infection and are composed of homotrimers of the pb1 protein. Pb1 has 1396 amino acids, of which the carboxy-terminal 133 residues form a trimeric intra-molecular chaperone that is auto-proteolyzed after correct folding. The structure of a trimer of residues 970–1263 was determined by single anomalous dispersion phasing using incorporated selenomethionine residues and refined at 2.3 Å resolution using crystals grown from native, methionine-containing, protein. The protein inhibits phage infection by competition. The phage-distal receptor-binding domain resembles a bullet, with the walls formed by partially intertwined beta-sheets, conferring stability to the structure. The fold of the domain is novel and the topology unique to the pb1 structure. A site-directed mutant (Ser1264 to Ala), in which auto-proteolysis is impeded, was also produced, crystallized and its 2.5 Å structure solved by molecular replacement. The additional chaperone domain (residues 1263–1396) consists of a central trimeric alpha-helical coiled-coil flanked by a mixed alpha-beta domain. Three long beta-hairpin tentacles, one from each chaperone monomer, extend into long curved grooves of the bullet-shaped domain. The chaperone-containing mutant did not inhibit infection by competition." @default.
- W2192991381 created "2016-06-24" @default.
- W2192991381 creator A5000040206 @default.
- W2192991381 creator A5000940279 @default.
- W2192991381 creator A5015817998 @default.
- W2192991381 creator A5017339188 @default.
- W2192991381 creator A5036389936 @default.
- W2192991381 creator A5055814623 @default.
- W2192991381 creator A5066658693 @default.
- W2192991381 creator A5066723649 @default.
- W2192991381 creator A5089499316 @default.
- W2192991381 date "2015-12-08" @default.
- W2192991381 modified "2023-10-18" @default.
- W2192991381 title "Structure of the Receptor-Binding Carboxy-Terminal Domain of the Bacteriophage T5 L-Shaped Tail Fibre with and without Its Intra-Molecular Chaperone" @default.
- W2192991381 cites W1484997386 @default.
- W2192991381 cites W1541191019 @default.
- W2192991381 cites W1620714331 @default.
- W2192991381 cites W1785835266 @default.
- W2192991381 cites W1891323639 @default.
- W2192991381 cites W1963733416 @default.
- W2192991381 cites W1965104216 @default.
- W2192991381 cites W1979376739 @default.
- W2192991381 cites W1980872605 @default.
- W2192991381 cites W1988664574 @default.
- W2192991381 cites W1989073611 @default.
- W2192991381 cites W1989987824 @default.
- W2192991381 cites W2007357231 @default.
- W2192991381 cites W2013586851 @default.
- W2192991381 cites W2013831456 @default.
- W2192991381 cites W2015425578 @default.
- W2192991381 cites W2016675163 @default.
- W2192991381 cites W2017692056 @default.
- W2192991381 cites W2018252076 @default.
- W2192991381 cites W2024256204 @default.
- W2192991381 cites W2030517878 @default.
- W2192991381 cites W2033060196 @default.
- W2192991381 cites W2035503835 @default.
- W2192991381 cites W2037106368 @default.
- W2192991381 cites W2037256626 @default.
- W2192991381 cites W2038193041 @default.
- W2192991381 cites W2051454056 @default.
- W2192991381 cites W2073949924 @default.
- W2192991381 cites W2076574570 @default.
- W2192991381 cites W2081272404 @default.
- W2192991381 cites W2086204994 @default.
- W2192991381 cites W2090360496 @default.
- W2192991381 cites W2101446086 @default.
- W2192991381 cites W2102580138 @default.
- W2192991381 cites W2108921801 @default.
- W2192991381 cites W2110808180 @default.
- W2192991381 cites W2122294312 @default.
- W2192991381 cites W2124026197 @default.
- W2192991381 cites W2127594598 @default.
- W2192991381 cites W2132629607 @default.
- W2192991381 cites W2134141380 @default.
- W2192991381 cites W2147949826 @default.
- W2192991381 cites W2154714625 @default.
- W2192991381 cites W2158860343 @default.
- W2192991381 cites W2159211495 @default.
- W2192991381 cites W2163341755 @default.
- W2192991381 cites W2171677744 @default.
- W2192991381 cites W2180229411 @default.
- W2192991381 cites W2214377940 @default.
- W2192991381 cites W2335620967 @default.
- W2192991381 cites W2412963709 @default.
- W2192991381 cites W53212497 @default.
- W2192991381 cites W66814248 @default.
- W2192991381 doi "https://doi.org/10.3390/v7122946" @default.
- W2192991381 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4690869" @default.
- W2192991381 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26670244" @default.
- W2192991381 hasPublicationYear "2015" @default.
- W2192991381 type Work @default.
- W2192991381 sameAs 2192991381 @default.
- W2192991381 citedByCount "42" @default.
- W2192991381 countsByYear W21929913812016 @default.
- W2192991381 countsByYear W21929913812017 @default.
- W2192991381 countsByYear W21929913812018 @default.
- W2192991381 countsByYear W21929913812019 @default.
- W2192991381 countsByYear W21929913812020 @default.
- W2192991381 countsByYear W21929913812021 @default.
- W2192991381 countsByYear W21929913812022 @default.
- W2192991381 countsByYear W21929913812023 @default.
- W2192991381 crossrefType "journal-article" @default.
- W2192991381 hasAuthorship W2192991381A5000040206 @default.
- W2192991381 hasAuthorship W2192991381A5000940279 @default.
- W2192991381 hasAuthorship W2192991381A5015817998 @default.
- W2192991381 hasAuthorship W2192991381A5017339188 @default.
- W2192991381 hasAuthorship W2192991381A5036389936 @default.
- W2192991381 hasAuthorship W2192991381A5055814623 @default.
- W2192991381 hasAuthorship W2192991381A5066658693 @default.
- W2192991381 hasAuthorship W2192991381A5066723649 @default.
- W2192991381 hasAuthorship W2192991381A5089499316 @default.
- W2192991381 hasBestOaLocation W21929913811 @default.
- W2192991381 hasConcept C104317684 @default.
- W2192991381 hasConcept C12554922 @default.
- W2192991381 hasConcept C142724271 @default.
- W2192991381 hasConcept C143065580 @default.
- W2192991381 hasConcept C166940927 @default.