FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2193609009> ?p ?o ?g. }

• W2193609009 endingPage "126" @default.
• W2193609009 startingPage "115" @default.
• W2193609009 abstract "Scintillation proximity assay (SPA) is a radio-isotopic technology format used to measure a wide range of biological interactions, including drug-target binding affinity studies. The assay is homogeneous in nature, as it relies on a mix and measure format. It does not involve a filtration step to separate bound from free ligand as is the case in a traditional receptor-binding assay. For G protein-coupled receptors (GPCRs), it has been shown that optimal binding kinetics, next to a high affinity of a ligand, can result in more desirable pharmacological profiles. However, traditional techniques to assess kinetic parameters tend to be cumbersome and laborious. We thus aimed to evaluate whether SPA can be an alternative platform for real-time receptor-binding kinetic measurements on GPCRs. To do so, we first validated the SPA technology for equilibrium binding studies on a prototypic class A GPCR, the human adenosine A1 receptor (hA1R). Differently to classic kinetic studies, the SPA technology allowed us to study binding kinetic processes almost real time, which is impossible in the filtration assay. To demonstrate the reliability of this technology for kinetic purposes, we performed the so-called competition association experiments. The association and dissociation rate constants (k on and k off) of unlabeled hA1R ligands were reliably and quickly determined and agreed very well with the same parameters from a traditional filtration assay performed simultaneously. In conclusion, SPA is a very promising technique to determine the kinetic profile of the drug-target interaction. Its robustness and potential for high-throughput may render this technology a preferred choice for further kinetic studies." @default.
• W2193609009 created "2016-06-24" @default.
• W2193609009 creator A5001540053 @default.
• W2193609009 creator A5038379459 @default.
• W2193609009 creator A5053401835 @default.
• W2193609009 creator A5065525958 @default.
• W2193609009 date "2015-12-09" @default.
• W2193609009 modified "2023-09-26" @default.
• W2193609009 title "Scintillation proximity assay (SPA) as a new approach to determine a ligand’s kinetic profile. A case in point for the adenosine A1 receptor" @default.
• W2193609009 cites W1562237695 @default.
• W2193609009 cites W1858777777 @default.
• W2193609009 cites W1892375967 @default.
• W2193609009 cites W1903983692 @default.
• W2193609009 cites W1959100396 @default.
• W2193609009 cites W1978227709 @default.
• W2193609009 cites W1985982430 @default.
• W2193609009 cites W1989146783 @default.
• W2193609009 cites W1991465998 @default.
• W2193609009 cites W2003366915 @default.
• W2193609009 cites W2003387609 @default.
• W2193609009 cites W2006774455 @default.
• W2193609009 cites W2019298705 @default.
• W2193609009 cites W2028245191 @default.
• W2193609009 cites W2030718808 @default.
• W2193609009 cites W2042963060 @default.
• W2193609009 cites W2047377985 @default.
• W2193609009 cites W2049650277 @default.
• W2193609009 cites W2052611207 @default.
• W2193609009 cites W2062205145 @default.
• W2193609009 cites W2074631079 @default.
• W2193609009 cites W2076643163 @default.
• W2193609009 cites W2094958614 @default.
• W2193609009 cites W2110614864 @default.
• W2193609009 cites W2111017095 @default.
• W2193609009 cites W2130212172 @default.
• W2193609009 cites W2135006542 @default.
• W2193609009 cites W2145982334 @default.
• W2193609009 cites W2149141089 @default.
• W2193609009 cites W2258399945 @default.
• W2193609009 cites W2893893333 @default.
• W2193609009 cites W3023234370 @default.
• W2193609009 cites W41987740 @default.
• W2193609009 cites W4232534952 @default.
• W2193609009 cites W2343850805 @default.
• W2193609009 doi "https://doi.org/10.1007/s11302-015-9485-0" @default.
• W2193609009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4749533" @default.
• W2193609009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26647040" @default.
• W2193609009 hasPublicationYear "2015" @default.
• W2193609009 type Work @default.
• W2193609009 sameAs 2193609009 @default.
• W2193609009 citedByCount "37" @default.
• W2193609009 countsByYear W21936090092016 @default.
• W2193609009 countsByYear W21936090092017 @default.
• W2193609009 countsByYear W21936090092018 @default.
• W2193609009 countsByYear W21936090092019 @default.
• W2193609009 countsByYear W21936090092020 @default.
• W2193609009 countsByYear W21936090092021 @default.
• W2193609009 countsByYear W21936090092022 @default.
• W2193609009 countsByYear W21936090092023 @default.
• W2193609009 crossrefType "journal-article" @default.
• W2193609009 hasAuthorship W2193609009A5001540053 @default.
• W2193609009 hasAuthorship W2193609009A5038379459 @default.
• W2193609009 hasAuthorship W2193609009A5053401835 @default.
• W2193609009 hasAuthorship W2193609009A5065525958 @default.
• W2193609009 hasBestOaLocation W21936090091 @default.
• W2193609009 hasConcept C107824862 @default.
• W2193609009 hasConcept C116569031 @default.
• W2193609009 hasConcept C121332964 @default.
• W2193609009 hasConcept C12554922 @default.
• W2193609009 hasConcept C135285700 @default.
• W2193609009 hasConcept C135889238 @default.
• W2193609009 hasConcept C159110408 @default.
• W2193609009 hasConcept C170493617 @default.
• W2193609009 hasConcept C185592680 @default.
• W2193609009 hasConcept C186060115 @default.
• W2193609009 hasConcept C2778938600 @default.
• W2193609009 hasConcept C41685203 @default.
• W2193609009 hasConcept C51323132 @default.
• W2193609009 hasConcept C55493867 @default.
• W2193609009 hasConcept C62520636 @default.
• W2193609009 hasConcept C67907053 @default.
• W2193609009 hasConcept C70721500 @default.
• W2193609009 hasConcept C71924100 @default.
• W2193609009 hasConcept C85196483 @default.
• W2193609009 hasConcept C86803240 @default.
• W2193609009 hasConceptScore W2193609009C107824862 @default.
• W2193609009 hasConceptScore W2193609009C116569031 @default.
• W2193609009 hasConceptScore W2193609009C121332964 @default.
• W2193609009 hasConceptScore W2193609009C12554922 @default.
• W2193609009 hasConceptScore W2193609009C135285700 @default.
• W2193609009 hasConceptScore W2193609009C135889238 @default.
• W2193609009 hasConceptScore W2193609009C159110408 @default.
• W2193609009 hasConceptScore W2193609009C170493617 @default.
• W2193609009 hasConceptScore W2193609009C185592680 @default.
• W2193609009 hasConceptScore W2193609009C186060115 @default.
• W2193609009 hasConceptScore W2193609009C2778938600 @default.
• W2193609009 hasConceptScore W2193609009C41685203 @default.
• W2193609009 hasConceptScore W2193609009C51323132 @default.

• next