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• W2194316238 abstract "The aim of this study was to use traditional mutagenesis to generate hyper-cellulolytic mutants with emphasis on stable, non-spore formers, shorter enzyme producing times and higher saccharification efficiency at high solid loadings. An in-house isolated strain of Aspergillus terreus (At) was identified, fingerprinted and mutated. A sequential process of mutation followed by stringent selection generated mutant At 9 , which produced optimal cellulase at day 4 instead of day 7, was non-spore former with high stability and grew on a lower pH than parental strain. At 9 cellulases were used successfully at high solid loads [up to 25 % (w/v)] in a modified system at 50 °C with reduced hydrolysis times compared to parent strain. In current work ultra violet (UV) mutagenesis and intelligent screening design combined with growth on a cheap substrate for enzyme production was demonstrated. With this work we present a single organism enzyme system with substantially lower production time and decreased saccharification time at high solid loads." @default.
• W2194316238 created "2016-06-24" @default.
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• W2194316238 date "2015-12-01" @default.
• W2194316238 modified "2023-09-25" @default.
• W2194316238 title "High solid saccharification using mild alkali-pretreated rice straw by hyper-cellulolytic fungal strain" @default.
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• W2194316238 doi "https://doi.org/10.1186/s40643-015-0075-3" @default.
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