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- W21956920 abstract "Phytoplasmas are routinely detected by nucleic acid-based techniques. These approaches rely on enriched phytoplasma DNA extracts of good quality, following labor intensive and time-consuming purification protocols. Here we describe a very rapid, specific, sensitive, and reliable method for flavescence dorée phytoplasma detection, based on real-time Taqman® reverse transcription-PCR of the 16S rRNA. The protocol is particularly useful for large-scale screening of vineyards and nurseries, pathogen surveys, and field epidemiological studies." @default.
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- W21956920 date "2012-08-19" @default.
- W21956920 modified "2023-09-23" @default.
- W21956920 title "Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions" @default.
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- W21956920 doi "https://doi.org/10.1007/978-1-62703-089-2_24" @default.
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