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- W219617632 abstract "A line of cultured human thyroid adenoma cells was used in a study designed to compare the stimulatory effect of TSH on cellular cAMP generation with the binding of radiolabeled TSH to the cells. At 37 C, specific binding of (/sup 125/I)TSH to suspensions of thyroid cells was maximal at 20 min and was reversed by the addition of excess TSH. Unlike the generation of cellular cAMP in response to TSH stimulation, which was maximal at pH 7.5, the binding of (/sup 125/)TSH to the cells was maximal at pH 5.5 and progressively declined up to pH 8.5. Increasing NaCl concentrations progressively inhibited cellular binding of TSH; at physiological salt concentrations, almost no TSH binding was detectable. Competitive inhibition studies of (/sup 125/I)TSH binding to cells revealed a binding site with a dissociation constant of 5.5 x 10/sup -8/ M at pH 7.4. GH, PRL, hCG, FSH, insulin, and glucagon did not compete with (/sup 125/I)TSH binding. ACTH, however, was a potent inhibitor of (/sup 125/I)TSH binding. Despite this inhibitory effect on TSH binding, ACTH had little or no effect on cellular cAMP generation. High concentrations of ACTH did not inhibit the biological effect of TSH on cAMP generation. Specificmore » binding of (/sup 125/I)TSH to empty plastic culture dishes was time dependent, reversible, and displayed a hormonal specificity identical to binding to thyroid cells. The effects of pH and NaCl concentrations on TSH binding to dishes were similarbut not identical to those on cellular binding. This study raises serious questions as to the biological significance of (/sup 125/I)TSH binding to cultured human thyroid cells.« less" @default.
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- W219617632 date "1978-12-01" @default.
- W219617632 modified "2023-09-23" @default.
- W219617632 title "Studies on binding of radiolabeled thyrotropin to cultured human thyroid cells. [/sup 125/I tracer technique]" @default.
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