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- W2196517295 abstract "Bacillus thuringiensis CAB530 was isolated from dead Anomala albopilosa (Rutelidae: Coleoptera) and soil of green tea field, and confirmed its insecticidal activities. CAB530 isolate showed a high insecticidal activity against the beet armyworm among the many lepidopteran insects that are difficult to control. LC?? value of CAB530 isolate against the second larva of Spodoptera exigua was 1.49×10⁴ spore concentration (cfu/㎖). SDS-PAGE result of insecticidal toxin protein of CAB530 isolate showed a band at 130 kDa that is similar pattern with B. thuringiensis subsp. kurstaki that took insecticidal activity against S. exigua. Otherwise, the crystal protein of the CAB530 isolate was conformed at 65 kDa level after 30 minute of incubation in S. exigua midgut juice. Six crystal genes (crylAa, crylAb, crylC, crylD, crylF and crylI) were identified by PCR. It different from genes of B. thuringiensis subsp. kurstaki. Crystal shape and pattern of toxin protein was similar with B. thuringiensis subsp. kurstaki, however, insecticidal activity and PCR result of CAB530 isolate was similar with B. thuringiensis subsp. aizawai." @default.
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- W2196517295 date "2011-06-01" @default.
- W2196517295 modified "2023-09-28" @default.
- W2196517295 title "청동풍뎅이에서 분리한 Bacillus thuringiensis CAB530 균주의 살충활성 및 분자학적 특성" @default.
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