Matches in SemOpenAlex for { <https://semopenalex.org/work/W2197786227> ?p ?o ?g. }
- W2197786227 endingPage "230" @default.
- W2197786227 startingPage "224" @default.
- W2197786227 abstract "Methods for protein characterization are being actively developed based on the growing importance of protein therapies and applications. The goal of this study was to demonstrate the use of size-exclusion chromatography (SEC) in combination with differential hydrogen-deuterium exchange (HDX) to compare protein global conformational changes at different solution conditions. Using chaotropic mobile phase additive, differential HDX was used to detect a number of solvent accessible labile protons of protein on-column at pH and temperature conditions which provided unrestricted intrinsic H/D exchange (all-or-nothing approach). Varying SEC on-column conditions allowed for protein conformational changes to be observed. Temperature and pressure were independently studied with regards to their effect on the proteins' (insulin, cytochrome C, ubiquitin, and myoglobin) conformational changes in the solution. The obtained ΔHDX profiles revealed protein conformational changes in solution under varied conditions manifested as the difference in the number of protons exchanged to deuterons, or vice-versa. The approach described in this manuscript could prove useful for protein batch-to-batch comparisons, for optimization of chemical reactions with enzyme as catalyst or for protein chemical modification reactions." @default.
- W2197786227 created "2016-06-24" @default.
- W2197786227 creator A5001016059 @default.
- W2197786227 creator A5007114172 @default.
- W2197786227 date "2016-01-01" @default.
- W2197786227 modified "2023-09-26" @default.
- W2197786227 title "Combining size-exclusion chromatography with differential hydrogen–deuterium exchange to study protein conformational changes" @default.
- W2197786227 cites W1533734182 @default.
- W2197786227 cites W1600015000 @default.
- W2197786227 cites W1966743338 @default.
- W2197786227 cites W1966895081 @default.
- W2197786227 cites W1969880312 @default.
- W2197786227 cites W1971014721 @default.
- W2197786227 cites W1974186522 @default.
- W2197786227 cites W1974429353 @default.
- W2197786227 cites W1980194012 @default.
- W2197786227 cites W1984291317 @default.
- W2197786227 cites W1984688081 @default.
- W2197786227 cites W1989646657 @default.
- W2197786227 cites W1996898871 @default.
- W2197786227 cites W2001647222 @default.
- W2197786227 cites W2001654431 @default.
- W2197786227 cites W2012434595 @default.
- W2197786227 cites W2014814117 @default.
- W2197786227 cites W2018312454 @default.
- W2197786227 cites W2022987595 @default.
- W2197786227 cites W2023993152 @default.
- W2197786227 cites W2024941839 @default.
- W2197786227 cites W2027461317 @default.
- W2197786227 cites W2029794817 @default.
- W2197786227 cites W2034878875 @default.
- W2197786227 cites W2036895460 @default.
- W2197786227 cites W2039873895 @default.
- W2197786227 cites W2052381685 @default.
- W2197786227 cites W2056764195 @default.
- W2197786227 cites W2058880472 @default.
- W2197786227 cites W2059918071 @default.
- W2197786227 cites W2061634691 @default.
- W2197786227 cites W2063811186 @default.
- W2197786227 cites W2065232574 @default.
- W2197786227 cites W2066859686 @default.
- W2197786227 cites W2068683917 @default.
- W2197786227 cites W2068831210 @default.
- W2197786227 cites W2070886775 @default.
- W2197786227 cites W2073001839 @default.
- W2197786227 cites W2075397811 @default.
- W2197786227 cites W2076667355 @default.
- W2197786227 cites W2078097318 @default.
- W2197786227 cites W2078984957 @default.
- W2197786227 cites W2094077114 @default.
- W2197786227 cites W2114078851 @default.
- W2197786227 cites W2114320763 @default.
- W2197786227 cites W2122680286 @default.
- W2197786227 cites W2136518039 @default.
- W2197786227 cites W2148013566 @default.
- W2197786227 cites W2152375064 @default.
- W2197786227 cites W2170036411 @default.
- W2197786227 cites W2332920806 @default.
- W2197786227 cites W2401776487 @default.
- W2197786227 cites W2951282694 @default.
- W2197786227 doi "https://doi.org/10.1016/j.chroma.2015.12.069" @default.
- W2197786227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26763301" @default.
- W2197786227 hasPublicationYear "2016" @default.
- W2197786227 type Work @default.
- W2197786227 sameAs 2197786227 @default.
- W2197786227 citedByCount "18" @default.
- W2197786227 countsByYear W21977862272016 @default.
- W2197786227 countsByYear W21977862272017 @default.
- W2197786227 countsByYear W21977862272019 @default.
- W2197786227 countsByYear W21977862272020 @default.
- W2197786227 countsByYear W21977862272021 @default.
- W2197786227 countsByYear W21977862272022 @default.
- W2197786227 crossrefType "journal-article" @default.
- W2197786227 hasAuthorship W2197786227A5001016059 @default.
- W2197786227 hasAuthorship W2197786227A5007114172 @default.
- W2197786227 hasConcept C102362939 @default.
- W2197786227 hasConcept C121332964 @default.
- W2197786227 hasConcept C162356407 @default.
- W2197786227 hasConcept C167569220 @default.
- W2197786227 hasConcept C181199279 @default.
- W2197786227 hasConcept C185592680 @default.
- W2197786227 hasConcept C193363816 @default.
- W2197786227 hasConcept C2778063876 @default.
- W2197786227 hasConcept C43617362 @default.
- W2197786227 hasConcept C47701112 @default.
- W2197786227 hasConcept C55493867 @default.
- W2197786227 hasConcept C58364064 @default.
- W2197786227 hasConcept C62520636 @default.
- W2197786227 hasConceptScore W2197786227C102362939 @default.
- W2197786227 hasConceptScore W2197786227C121332964 @default.
- W2197786227 hasConceptScore W2197786227C162356407 @default.
- W2197786227 hasConceptScore W2197786227C167569220 @default.
- W2197786227 hasConceptScore W2197786227C181199279 @default.
- W2197786227 hasConceptScore W2197786227C185592680 @default.
- W2197786227 hasConceptScore W2197786227C193363816 @default.
- W2197786227 hasConceptScore W2197786227C2778063876 @default.
- W2197786227 hasConceptScore W2197786227C43617362 @default.
- W2197786227 hasConceptScore W2197786227C47701112 @default.