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- W2197957669 abstract "(19)F solution NMR is a powerful and versatile tool to study protein structure and protein-ligand interactions due to the favorable NMR characteristics of the (19)F atom, its absence in naturally occurring biomolecules, and small size. Protocols to introduce (19)F atoms into both proteins and their ligands are readily available and offer the ability to conduct protein-observe (using (19)F-labeled proteins) or ligand-observe (using (19)F-containing ligands) NMR experiments. This chapter provides two protocols for the (19)F-labeling of proteins, using an Escherichia coli expression system: (i) amino acid type-specific incorporation of (19)F-modified amino acids and (ii) site-specific incorporation of (19)F-modified amino acids using recombinantly expressed orthogonal amber tRNA/tRNA synthetase pairs. In addition, we discuss several applications, involving (19)F-modified proteins and (19)F-containing ligands." @default.
- W2197957669 created "2016-06-24" @default.
- W2197957669 creator A5004995359 @default.
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- W2197957669 date "2015-01-01" @default.
- W2197957669 modified "2023-10-01" @default.
- W2197957669 title "19F-Modified Proteins and 19F-Containing Ligands as Tools in Solution NMR Studies of Protein Interactions" @default.
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- W2197957669 doi "https://doi.org/10.1016/bs.mie.2015.05.014" @default.
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