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• W2198603666 abstract "In this study, we evaluated trimethylsilyl (TMS) derivatives as derivatization reagents for the compound-specific stable carbon isotope analysis of soil amino acids by gas chromatography–combustion–isotope ratio mass spectrometry (GC–C–IRMS). We used non-proteinogenic amino acids to show that the extraction–derivatization–analysis procedure provides a reliable method to measure δ13C values of amino acids extracted from soil. However, we found a number of drawbacks that significantly increase the final total uncertainty. These include the following: production of multiple peaks for each amino acid, identified as di-, tri- and tetra-TMS derivatives;a number of TMS-carbon (TMS-C) atoms added lower than the stoichiometric one, possibly due to incomplete combustion;different TMS-C δ13C for di-, tri- and tetra-TMS derivatives. For soil samples, only four amino acids (leucine, valine, threonine and serine) provide reliable δ13C values with a total average uncertainty of 1.3 ‰. We conclude that trimethylsilyl derivatives are only suitable for determining the 13C incorporation in amino acids within experiments using 13C-labelled tracers but cannot be applied for amino acids with natural carbon isotope abundance until the drawbacks described here are overcome and the measured total uncertainty significantly decreased." @default.
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• W2198603666 date "2014-10-02" @default.
• W2198603666 modified "2023-09-25" @default.
• W2198603666 title "Measurement of δ¹³C values of soil amino acids by GC–C–IRMS using trimethylsilylation: a critical assessment" @default.
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• W2198603666 doi "https://doi.org/10.1080/10256016.2014.959444" @default.
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