Matches in SemOpenAlex for { <https://semopenalex.org/work/W2200325631> ?p ?o ?g. }
- W2200325631 endingPage "12710" @default.
- W2200325631 startingPage "12705" @default.
- W2200325631 abstract "Single cardiomyocytes contain myofibrils that harbor the sarcomere-based contractile machinery of the myocardium. Cardiomyocytes differentiated from human pluripotent stem cells (hPSC-CMs) have potential as an in vitro model of heart activity. However, their fetal-like misalignment of myofibrils limits their usefulness for modeling contractile activity. We analyzed the effects of cell shape and substrate stiffness on the shortening and movement of labeled sarcomeres and the translation of sarcomere activity to mechanical output (contractility) in live engineered hPSC-CMs. Single hPSC-CMs were cultured on polyacrylamide substrates of physiological stiffness (10 kPa), and Matrigel micropatterns were used to generate physiological shapes (2,000-µm(2) rectangles with length:width aspect ratios of 5:1-7:1) and a mature alignment of myofibrils. Translation of sarcomere shortening to mechanical output was highest in 7:1 hPSC-CMs. Increased substrate stiffness and applied overstretch induced myofibril defects in 7:1 hPSC-CMs and decreased mechanical output. Inhibitors of nonmuscle myosin activity repressed the assembly of myofibrils, showing that subcellular tension drives the improved contractile activity in these engineered hPSC-CMs. Other factors associated with improved contractility were axially directed calcium flow, systematic mitochondrial distribution, more mature electrophysiology, and evidence of transverse-tubule formation. These findings support the potential of these engineered hPSC-CMs as powerful models for studying myocardial contractility at the cellular level." @default.
- W2200325631 created "2016-06-24" @default.
- W2200325631 creator A5000381401 @default.
- W2200325631 creator A5001995249 @default.
- W2200325631 creator A5005728106 @default.
- W2200325631 creator A5014077740 @default.
- W2200325631 creator A5019139892 @default.
- W2200325631 creator A5038493219 @default.
- W2200325631 creator A5042133998 @default.
- W2200325631 creator A5052283727 @default.
- W2200325631 date "2015-09-28" @default.
- W2200325631 modified "2023-10-18" @default.
- W2200325631 title "Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness" @default.
- W2200325631 cites W1688654027 @default.
- W2200325631 cites W1964758465 @default.
- W2200325631 cites W1966140207 @default.
- W2200325631 cites W1966543040 @default.
- W2200325631 cites W1968272833 @default.
- W2200325631 cites W1971163936 @default.
- W2200325631 cites W1973089587 @default.
- W2200325631 cites W1975538320 @default.
- W2200325631 cites W1981347990 @default.
- W2200325631 cites W1985617348 @default.
- W2200325631 cites W1989013698 @default.
- W2200325631 cites W1991043063 @default.
- W2200325631 cites W1998849298 @default.
- W2200325631 cites W2000381214 @default.
- W2200325631 cites W2002594335 @default.
- W2200325631 cites W2005496211 @default.
- W2200325631 cites W2010020475 @default.
- W2200325631 cites W2010081173 @default.
- W2200325631 cites W2020203296 @default.
- W2200325631 cites W2029971013 @default.
- W2200325631 cites W2030600914 @default.
- W2200325631 cites W2033765226 @default.
- W2200325631 cites W2039276611 @default.
- W2200325631 cites W2046412711 @default.
- W2200325631 cites W2048277552 @default.
- W2200325631 cites W2049181319 @default.
- W2200325631 cites W2049566642 @default.
- W2200325631 cites W2058846791 @default.
- W2200325631 cites W2067275294 @default.
- W2200325631 cites W2068643393 @default.
- W2200325631 cites W2085950714 @default.
- W2200325631 cites W2086307279 @default.
- W2200325631 cites W2087625365 @default.
- W2200325631 cites W2094121810 @default.
- W2200325631 cites W2094160006 @default.
- W2200325631 cites W2096151652 @default.
- W2200325631 cites W2097848460 @default.
- W2200325631 cites W2098082531 @default.
- W2200325631 cites W2098188264 @default.
- W2200325631 cites W2100541308 @default.
- W2200325631 cites W2101274334 @default.
- W2200325631 cites W2101609952 @default.
- W2200325631 cites W2102121146 @default.
- W2200325631 cites W2103446573 @default.
- W2200325631 cites W2103495097 @default.
- W2200325631 cites W2105072296 @default.
- W2200325631 cites W2114879284 @default.
- W2200325631 cites W2122140247 @default.
- W2200325631 cites W2130193373 @default.
- W2200325631 cites W2134722345 @default.
- W2200325631 cites W2137542902 @default.
- W2200325631 cites W2140452645 @default.
- W2200325631 cites W2144916644 @default.
- W2200325631 cites W2148328620 @default.
- W2200325631 cites W2148583233 @default.
- W2200325631 cites W2148693232 @default.
- W2200325631 cites W2151492748 @default.
- W2200325631 cites W2157023104 @default.
- W2200325631 cites W2159111743 @default.
- W2200325631 cites W2161390320 @default.
- W2200325631 cites W2162365100 @default.
- W2200325631 cites W2163824934 @default.
- W2200325631 cites W2164232745 @default.
- W2200325631 cites W2169011454 @default.
- W2200325631 cites W2326648613 @default.
- W2200325631 cites W339121283 @default.
- W2200325631 cites W95923283 @default.
- W2200325631 doi "https://doi.org/10.1073/pnas.1508073112" @default.
- W2200325631 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4611612" @default.
- W2200325631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26417073" @default.
- W2200325631 hasPublicationYear "2015" @default.
- W2200325631 type Work @default.
- W2200325631 sameAs 2200325631 @default.
- W2200325631 citedByCount "349" @default.
- W2200325631 countsByYear W22003256312016 @default.
- W2200325631 countsByYear W22003256312017 @default.
- W2200325631 countsByYear W22003256312018 @default.
- W2200325631 countsByYear W22003256312019 @default.
- W2200325631 countsByYear W22003256312020 @default.
- W2200325631 countsByYear W22003256312021 @default.
- W2200325631 countsByYear W22003256312022 @default.
- W2200325631 countsByYear W22003256312023 @default.
- W2200325631 crossrefType "journal-article" @default.
- W2200325631 hasAuthorship W2200325631A5000381401 @default.
- W2200325631 hasAuthorship W2200325631A5001995249 @default.