FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2200326564> ?p ?o ?g. }

• W2200326564 endingPage "21" @default.
• W2200326564 startingPage "11" @default.
• W2200326564 abstract "Development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. This study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with E1A 13S protein on human tissue plasminogen activator (t-PA) expression in Chinese hamster ovary (CHO) cells. Methods: The matrix attachment region was cloned in 3΄ and 5΄ flanking sides of the t- PA expression cassette in pTPA vector to generate pMTPA. After transfection of the cells with pTPA and pMTPA vectors, stable cell pools were developed and the t-PA expression level determined for each stable cell line. In the next step, E1A 13S expression plasmid was transfected to stable cell pools and t-PA titers were measured after 72 hours. Results: Integration of pTPA and pMTPA vectors in the CHO genome was confirmed by PCR analysis on genomic DNA of stable cell pools. Analysis of the t-PA expression level showed a three-fold enhancement in pMTPA transfected cells compared to pTPA- containing cells. t-PA expression was further enhanced up to 1771 U/ml by transient expression of E1A 13S in pMTPA stable cell pools. Conclusion: These results have shown that incorporation of matrix attachment region in an expression vector in combination with promoter activation can effectively enhance recombinant protein expression levels in CHO cells." @default.
• W2200326564 created "2016-06-24" @default.
• W2200326564 creator A5011350693 @default.
• W2200326564 creator A5014275960 @default.
• W2200326564 creator A5028326676 @default.
• W2200326564 creator A5035771210 @default.
• W2200326564 creator A5039363303 @default.
• W2200326564 creator A5089827462 @default.
• W2200326564 date "2013-01-01" @default.
• W2200326564 modified "2023-09-23" @default.
• W2200326564 title "ENHANCEMENT OF RECOMBINANT HUMAN TISSUE PLASMINOGEN ACTIVATOR EXPRESSION IN CHO CELLS USING MATRIX ATTACHMENT REGION CONTAINING VECTORS IN COMBINATION WITH PROMOTER ACTIVATION STRATEGY" @default.
• W2200326564 cites W1909000005 @default.
• W2200326564 cites W1967240723 @default.
• W2200326564 cites W1972007952 @default.
• W2200326564 cites W1984188931 @default.
• W2200326564 cites W2006554573 @default.
• W2200326564 cites W2012034770 @default.
• W2200326564 cites W2029091816 @default.
• W2200326564 cites W2038431234 @default.
• W2200326564 cites W2041472143 @default.
• W2200326564 cites W2049610944 @default.
• W2200326564 cites W2053794026 @default.
• W2200326564 cites W2068522955 @default.
• W2200326564 cites W2074272255 @default.
• W2200326564 cites W2078618801 @default.
• W2200326564 cites W2087007992 @default.
• W2200326564 cites W2097536972 @default.
• W2200326564 cites W2114425910 @default.
• W2200326564 cites W2118540273 @default.
• W2200326564 cites W2139701002 @default.
• W2200326564 cites W2146841456 @default.
• W2200326564 cites W2150582853 @default.
• W2200326564 cites W2157545440 @default.
• W2200326564 cites W2166613267 @default.
• W2200326564 hasPublicationYear "2013" @default.
• W2200326564 type Work @default.
• W2200326564 sameAs 2200326564 @default.
• W2200326564 citedByCount "0" @default.
• W2200326564 crossrefType "journal-article" @default.
• W2200326564 hasAuthorship W2200326564A5011350693 @default.
• W2200326564 hasAuthorship W2200326564A5014275960 @default.
• W2200326564 hasAuthorship W2200326564A5028326676 @default.
• W2200326564 hasAuthorship W2200326564A5035771210 @default.
• W2200326564 hasAuthorship W2200326564A5039363303 @default.
• W2200326564 hasAuthorship W2200326564A5089827462 @default.
• W2200326564 hasConcept C100012643 @default.
• W2200326564 hasConcept C104317684 @default.
• W2200326564 hasConcept C150194340 @default.
• W2200326564 hasConcept C153911025 @default.
• W2200326564 hasConcept C167227067 @default.
• W2200326564 hasConcept C175656101 @default.
• W2200326564 hasConcept C185592680 @default.
• W2200326564 hasConcept C22744801 @default.
• W2200326564 hasConcept C33161422 @default.
• W2200326564 hasConcept C40767141 @default.
• W2200326564 hasConcept C54009773 @default.
• W2200326564 hasConcept C54355233 @default.
• W2200326564 hasConcept C55493867 @default.
• W2200326564 hasConcept C56262557 @default.
• W2200326564 hasConcept C81885089 @default.
• W2200326564 hasConcept C86803240 @default.
• W2200326564 hasConcept C92087593 @default.
• W2200326564 hasConcept C95444343 @default.
• W2200326564 hasConceptScore W2200326564C100012643 @default.
• W2200326564 hasConceptScore W2200326564C104317684 @default.
• W2200326564 hasConceptScore W2200326564C150194340 @default.
• W2200326564 hasConceptScore W2200326564C153911025 @default.
• W2200326564 hasConceptScore W2200326564C167227067 @default.
• W2200326564 hasConceptScore W2200326564C175656101 @default.
• W2200326564 hasConceptScore W2200326564C185592680 @default.
• W2200326564 hasConceptScore W2200326564C22744801 @default.
• W2200326564 hasConceptScore W2200326564C33161422 @default.
• W2200326564 hasConceptScore W2200326564C40767141 @default.
• W2200326564 hasConceptScore W2200326564C54009773 @default.
• W2200326564 hasConceptScore W2200326564C54355233 @default.
• W2200326564 hasConceptScore W2200326564C55493867 @default.
• W2200326564 hasConceptScore W2200326564C56262557 @default.
• W2200326564 hasConceptScore W2200326564C81885089 @default.
• W2200326564 hasConceptScore W2200326564C86803240 @default.
• W2200326564 hasConceptScore W2200326564C92087593 @default.
• W2200326564 hasConceptScore W2200326564C95444343 @default.
• W2200326564 hasIssue "1" @default.
• W2200326564 hasLocation W22003265641 @default.
• W2200326564 hasOpenAccess W2200326564 @default.
• W2200326564 hasPrimaryLocation W22003265641 @default.
• W2200326564 hasRelatedWork W1972448672 @default.
• W2200326564 hasRelatedWork W2041064967 @default.
• W2200326564 hasRelatedWork W2051657069 @default.
• W2200326564 hasRelatedWork W2073729403 @default.
• W2200326564 hasRelatedWork W2091410421 @default.
• W2200326564 hasRelatedWork W2125396741 @default.
• W2200326564 hasRelatedWork W2137538430 @default.
• W2200326564 hasRelatedWork W2157392441 @default.
• W2200326564 hasRelatedWork W2348428835 @default.
• W2200326564 hasRelatedWork W2348716154 @default.
• W2200326564 hasRelatedWork W2357812082 @default.
• W2200326564 hasRelatedWork W2361524283 @default.
• W2200326564 hasRelatedWork W2361721561 @default.

• next