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- W2200348536 abstract "Publisher Summary This chapter describes the assay, purification, and properties of shikimate dehydrogenase. The enzyme is assayed in the direction of shikimate oxidation by following the production of nicotinamide adenine dinucleotide phosphate (NADPH) spectrophotometrically at 340 m μ . Coupling the reaction with glutathione disulfide (GSSG) reductase is not necessary, as NADPH production is linear for at least five minutes. A unit of activity is the amount of enzyme catalyzing the production of 1 micromole of NADPH per minute, assuming a molar extinction coefficient for NADPH of 6.22 × 10 3 . Protein is determined by measuring the optical density at 280 m μ , assuming a protein concentration of 1 mg/ml to give an optical density reading of 1. The assay procedure can be used with crude preparations, provided a blank cuvette containing all reagents except shikimate is used as control. The purification procedure essentially provides an enzyme preparation of higher purity and greater stability. The presence of cysteine in the buffers protects the enzyme from inactivation." @default.
- W2200348536 created "2016-06-24" @default.
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- W2200348536 date "1970-01-01" @default.
- W2200348536 modified "2023-09-27" @default.
- W2200348536 title "[43c] Shikimate dehydrogenase1–2 (peas)" @default.
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- W2200348536 doi "https://doi.org/10.1016/0076-6879(71)17212-6" @default.
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