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- W2200507237 abstract "l-Phenyllactic acid (l-PLA) is a novel antiseptic agent with broad and effective antimicrobial activity. In addition, l-PLA has been used for synthesis of poly(phenyllactic acid)s, which exhibits better mechanical properties than poly(lactic acid)s. However, the concentration and optical purity of l-PLA produced by native microbes was rather low. An NAD-dependent l-lactate dehydrogenase (l-nLDH) from Bacillus coagulans NL01 was confirmed to have a good ability to produce l-PLA from phenylpyruvic acid (PPA). In the present study, l-nLDH gene and formate dehydrogenase gene were heterologously coexpressed in Escherichia coli. Through two coupled reactions, 79.6 mM l-PLA was produced from 82.8 mM PPA in 40 min and the enantiomeric excess value of l-PLA was high (>99%). Therefore, this process suggested a promising alternative for the production of chiral l-PLA." @default.
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- W2200507237 date "2015-08-01" @default.
- W2200507237 modified "2023-09-23" @default.
- W2200507237 title "The added value of thermally assisted high pressure processing in obtaining probiotic yogurt from cow & goat milk" @default.
- W2200507237 doi "https://doi.org/10.1016/j.jbiotec.2015.06.137" @default.
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