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- W2200580347 abstract "Nucleotide arrays require controlled surface densities and minimal nucleotide-substrate interactions to enable highly specific and efficient recognition by corresponding targets. We investigated chemical lift-off lithography with hydroxyl- and oligo(ethylene glycol)-terminated alkanethiol self-assembled monolayers as a means to produce substrates optimized for tethered DNA insertion into post-lift-off regions. Residual alkanethiols in the patterned regions after lift-off lithography enabled the formation of patterned DNA monolayers that favored hybridization with target DNA. Nucleotide densities were tunable by altering surface chemistries and alkanethiol ratios prior to lift-off. Lithography-induced conformational changes in oligo(ethylene glycol)-terminated monolayers hindered nucleotide insertion but could be used to advantage via mixed monolayers or double-lift-off lithography. Compared to thiolated DNA self-assembly alone or with alkanethiol backfilling, preparation of functional nucleotide arrays by chemical lift-off lithography enables superior hybridization efficiency and tunability." @default.
- W2200580347 created "2016-06-24" @default.
- W2200580347 creator A5001219858 @default.
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- W2200580347 creator A5079192531 @default.
- W2200580347 date "2015-10-06" @default.
- W2200580347 modified "2023-09-25" @default.
- W2200580347 title "Controlled DNA Patterning by Chemical Lift-Off Lithography: Matrix Matters" @default.
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- W2200580347 doi "https://doi.org/10.1021/acsnano.5b05546" @default.
- W2200580347 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26426585" @default.
- W2200580347 hasPublicationYear "2015" @default.