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• W2200596311 abstract "Despite advances in diagnostics and treatments, many cancer patients have poor survival rates. Tumours develop drug resistance followed by metastasis, and survivors suffer from treatment side-effects. Omics techniques, targeted treatments and immunotherapy offer the prospect of individually adapting treatments for optimal efficacy and minimal side-effects. This requires integration of biomolecular, clinical and drug data to successfully predict optimal treatments for every patient. The aim of this thesis was to evaluate and apply different methodologies important for personalised treatment in a variety of cancer settings. The first paper showed that E6/E7 mRNA detection through RT-NASBA is more accurate and sensitive than DNA genotyping for classifying HPV infection in cervical adenocarcinoma, showing RNA analysis to be preferable for identifying high-risk patients. In paper II, HPV16 E2 and E5 mRNA expression in oropharyngeal cancer was analysed in relation to clinical outcomes and tumour immunology. Neither down-regulation of HLA class I nor CD8 + T-cell infiltration, both indicators of good prognosis, were dependent on E2 or E5. However, absence of E2 was related to poor progression-free survival. This allows E2 expression to be combined with HLA class I and CD8 + T-cells when stratifying patients with good prognosis for milder treatment. The third paper screened combinations of growth factors and drugs for impact on proliferation of breast cancer cells, creating a two-dimensional space to simulate tumours in different signalling states interacting with drugs. In MDA-MB-231 cells, TGF-β in combination with EGF and oestrogen inhibited growth, with the effect strengthened by Tamoxifen. In MCF7 cells, Tamoxifen inhibited growth when added to both EGF and oestrogen. In paper IV, the immunoproteome in urinary bladder cancer was analysed. Proteomics and network analysis of regulatory (Treg) and effector T-cells (Teff) of lymph nodes showed that Tregs in sentinel nodes (SN) up-regulate growth and immune signalling networks. IL16, previously not shown to be expressed by Tregs, was predicted as central to SN-Treg signalling. IL-16 expression in Tregs was validated, shown to be higher in lymph nodes than peripheral blood and inhibited by tumour cell supernatant. In conclusion, this thesis has shown methods to improve patient stratification in cervical adenocarcinoma and HPV-positive oropharyngeal cancer. The utility of proliferation screenings replicating tumour heterogeneity for optimising drug combinations was demonstrated, and finally, lymph node proteomics revealed individual differences in T-cell signalling, important for optimising immunotherapy. Integration of these and other methods will be key to arrive at personalised cancer medicine – application of the optimal treatment combination for every patient. LIST OF SCIENTIFIC PAPERS I. Hovland S, Muller S, Skomedal H, Mints M, Bergstrom J, Wallin KL, Karlsen F, Johansson B, Andersson S. E6/E7 mRNA expression analysis: a test for the objective assessment of cervical adenocarcinoma in clinical prognostic procedure. Int J Oncol. 2010 Jun;36(6):1533-9. II. Ramqvist T * , Mints M * , Tertipis N, Nasman A, Romanitan М, MunckWikland В, Dalianis T. Studies on Human Papillomavirus (HPV) 16 E2, E5 and E7 mRNA in HPV-positive Tonsillar and Base of Tongue Cancer in Relation to Clinical Outcome and Immunological Parameters. Oral Oncology (In press). III. Mints M, Souchelnytskyi S. Impact of combinations of EGF, TGFβ, 17βoestradiol, and inhibitors of corresponding pathways on proliferation of breast cancer cell lines. Exp Oncol. 2014 Jun;36(2):67-71. IV. Mints M, Krantz D, Winerdal М, Rutishauser D, Zubarev R, Zirakzadeh АA, Hansson J, Sherif A, Winqvist О. Individual proteomics on T-cell subsets in muscle-invasive urothelial bladder cancer patient lymph nodes reveals IL-16 as a central player in tumour-Treg cross-talk. (Manuscript). *Shared first authorship CONTENTS" @default.
• W2200596311 created "2016-06-24" @default.
• W2200596311 creator A5046093053 @default.
• W2200596311 date "2015-10-02" @default.
• W2200596311 modified "2023-09-23" @default.
• W2200596311 title "Methodological Approaches towards Personalised Cancer Medicine" @default.
• W2200596311 cites W1026305928 @default.
• W2200596311 cites W110793174 @default.
• W2200596311 cites W141770798 @default.
• W2200596311 cites W142237009 @default.
• W2200596311 cites W1499240675 @default.
• W2200596311 cites W1517051009 @default.
• W2200596311 cites W1555993773 @default.
• W2200596311 cites W1757407923 @default.
• W2200596311 cites W1813571362 @default.
• W2200596311 cites W1964363300 @default.
• W2200596311 cites W1964461587 @default.
• W2200596311 cites W1965707365 @default.
• W2200596311 cites W1965709519 @default.
• W2200596311 cites W1968396632 @default.
• W2200596311 cites W1973571261 @default.
• W2200596311 cites W1973856794 @default.
• W2200596311 cites W1977190067 @default.
• W2200596311 cites W1979648005 @default.
• W2200596311 cites W1981989535 @default.
• W2200596311 cites W1983670437 @default.
• W2200596311 cites W1987704443 @default.
• W2200596311 cites W1988055795 @default.
• W2200596311 cites W1990085600 @default.
• W2200596311 cites W1992025025 @default.
• W2200596311 cites W1992708996 @default.
• W2200596311 cites W1992778754 @default.
• W2200596311 cites W1996216604 @default.
• W2200596311 cites W1996398553 @default.
• W2200596311 cites W1996778928 @default.
• W2200596311 cites W1997538260 @default.
• W2200596311 cites W1997740904 @default.
• W2200596311 cites W1997890663 @default.
• W2200596311 cites W1998728654 @default.
• W2200596311 cites W1999403469 @default.
• W2200596311 cites W1999506279 @default.
• W2200596311 cites W2001258296 @default.
• W2200596311 cites W2002362032 @default.
• W2200596311 cites W2005582084 @default.
• W2200596311 cites W2008959658 @default.
• W2200596311 cites W2011152729 @default.
• W2200596311 cites W2012124753 @default.
• W2200596311 cites W2014985358 @default.
• W2200596311 cites W2017007251 @default.
• W2200596311 cites W2017046792 @default.
• W2200596311 cites W2017577544 @default.
• W2200596311 cites W2017693082 @default.
• W2200596311 cites W2018312170 @default.
• W2200596311 cites W2018710935 @default.
• W2200596311 cites W2019270618 @default.
• W2200596311 cites W2019665726 @default.
• W2200596311 cites W2020920166 @default.
• W2200596311 cites W2025635023 @default.
• W2200596311 cites W2028084128 @default.
• W2200596311 cites W2028541216 @default.
• W2200596311 cites W2028897202 @default.
• W2200596311 cites W2028959752 @default.
• W2200596311 cites W2029660804 @default.
• W2200596311 cites W2030182616 @default.
• W2200596311 cites W2030259772 @default.
• W2200596311 cites W2033346155 @default.
• W2200596311 cites W2035020230 @default.
• W2200596311 cites W2035252444 @default.
• W2200596311 cites W2035297454 @default.
• W2200596311 cites W2039674259 @default.
• W2200596311 cites W2039722727 @default.
• W2200596311 cites W2040458067 @default.
• W2200596311 cites W2041949509 @default.
• W2200596311 cites W2041993682 @default.
• W2200596311 cites W2043361233 @default.
• W2200596311 cites W2043671365 @default.
• W2200596311 cites W2043904711 @default.
• W2200596311 cites W2044433401 @default.
• W2200596311 cites W2046482090 @default.
• W2200596311 cites W2048787584 @default.
• W2200596311 cites W2052025125 @default.
• W2200596311 cites W2053765305 @default.
• W2200596311 cites W2059476598 @default.
• W2200596311 cites W2060070314 @default.
• W2200596311 cites W2061061337 @default.
• W2200596311 cites W2063477624 @default.
• W2200596311 cites W2063532871 @default.
• W2200596311 cites W2064561270 @default.
• W2200596311 cites W2066621103 @default.
• W2200596311 cites W2066722284 @default.
• W2200596311 cites W2068523052 @default.
• W2200596311 cites W2070040136 @default.
• W2200596311 cites W2072010297 @default.
• W2200596311 cites W2072810564 @default.
• W2200596311 cites W2073332412 @default.
• W2200596311 cites W2073417582 @default.
• W2200596311 cites W2074737893 @default.
• W2200596311 cites W2075780501 @default.
• W2200596311 cites W2076951669 @default.
• W2200596311 cites W2079563665 @default.

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