FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2201254124> ?p ?o ?g. }

• W2201254124 endingPage "239" @default.
• W2201254124 startingPage "221" @default.
• W2201254124 abstract "Measuring changes in intracellular Ca2+ concentration ([Ca2+] i ) with optical methods is a useful approach to study the function and regulation of Ca2+-permeable ion channels, such as ionotropic glutamate receptors. Here we describe a practical method for monitoring changes in [Ca2+] i that employs the widely used Ca2+ indicator, fura-2. Upon binding Ca2+, the excitation maximum of fura-2 shifts from 380 to 340 nm. Therefore, the ratio of fura-2 fluorescence from cells excited at 340 relative to 380 nm tracks changes in [Ca2+] i ; importantly this ratio is independent of dye concentration, optical path length, or illumination intensity. We provide instructions for calibrating an imaging system and using ratio-metric analysis for processing fura-2 fluorescence images to represent [Ca2+] i . Common technical problems are discussed in a section devoted to troubleshooting. Fura-2-based digital imaging has become a widely used technique with broad applicability. We describe methods to accomplish particularly common [Ca2+] i imaging goals; however, these provide a versatile foundation that can be further developed into more complex approaches to acquire [Ca2+] i -dependent images with higher temporal or spatial resolution, and from more challenging preparations." @default.
• W2201254124 created "2016-06-24" @default.
• W2201254124 creator A5021569126 @default.
• W2201254124 creator A5038294089 @default.
• W2201254124 date "2016-01-01" @default.
• W2201254124 modified "2023-09-27" @default.
• W2201254124 title "Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses" @default.
• W2201254124 cites W1571164133 @default.
• W2201254124 cites W1818623226 @default.
• W2201254124 cites W18639822 @default.
• W2201254124 cites W1970657787 @default.
• W2201254124 cites W2006216368 @default.
• W2201254124 cites W2023175942 @default.
• W2201254124 cites W2023200476 @default.
• W2201254124 cites W2032562864 @default.
• W2201254124 cites W2034070803 @default.
• W2201254124 cites W2041416738 @default.
• W2201254124 cites W2043972280 @default.
• W2201254124 cites W2053559739 @default.
• W2201254124 cites W2068321659 @default.
• W2201254124 cites W2072578266 @default.
• W2201254124 cites W2072953011 @default.
• W2201254124 cites W2100446252 @default.
• W2201254124 cites W2127166394 @default.
• W2201254124 cites W2136095861 @default.
• W2201254124 cites W2141260882 @default.
• W2201254124 cites W2144136652 @default.
• W2201254124 cites W2168979459 @default.
• W2201254124 cites W2239360134 @default.
• W2201254124 cites W340599019 @default.
• W2201254124 cites W4237030338 @default.
• W2201254124 doi "https://doi.org/10.1007/978-1-4939-2812-5_15" @default.
• W2201254124 hasPublicationYear "2016" @default.
• W2201254124 type Work @default.
• W2201254124 sameAs 2201254124 @default.
• W2201254124 citedByCount "1" @default.
• W2201254124 countsByYear W22012541242018 @default.
• W2201254124 crossrefType "book-chapter" @default.
• W2201254124 hasAuthorship W2201254124A5021569126 @default.
• W2201254124 hasAuthorship W2201254124A5038294089 @default.
• W2201254124 hasConcept C111919701 @default.
• W2201254124 hasConcept C113196181 @default.
• W2201254124 hasConcept C115955781 @default.
• W2201254124 hasConcept C120665830 @default.
• W2201254124 hasConcept C121332964 @default.
• W2201254124 hasConcept C12554922 @default.
• W2201254124 hasConcept C147494362 @default.
• W2201254124 hasConcept C170493617 @default.
• W2201254124 hasConcept C178790620 @default.
• W2201254124 hasConcept C181199279 @default.
• W2201254124 hasConcept C185592680 @default.
• W2201254124 hasConcept C186060115 @default.
• W2201254124 hasConcept C2776032975 @default.
• W2201254124 hasConcept C2778470358 @default.
• W2201254124 hasConcept C41008148 @default.
• W2201254124 hasConcept C43617362 @default.
• W2201254124 hasConcept C519063684 @default.
• W2201254124 hasConcept C55493867 @default.
• W2201254124 hasConcept C61174792 @default.
• W2201254124 hasConcept C86803240 @default.
• W2201254124 hasConcept C91881484 @default.
• W2201254124 hasConcept C98539663 @default.
• W2201254124 hasConceptScore W2201254124C111919701 @default.
• W2201254124 hasConceptScore W2201254124C113196181 @default.
• W2201254124 hasConceptScore W2201254124C115955781 @default.
• W2201254124 hasConceptScore W2201254124C120665830 @default.
• W2201254124 hasConceptScore W2201254124C121332964 @default.
• W2201254124 hasConceptScore W2201254124C12554922 @default.
• W2201254124 hasConceptScore W2201254124C147494362 @default.
• W2201254124 hasConceptScore W2201254124C170493617 @default.
• W2201254124 hasConceptScore W2201254124C178790620 @default.
• W2201254124 hasConceptScore W2201254124C181199279 @default.
• W2201254124 hasConceptScore W2201254124C185592680 @default.
• W2201254124 hasConceptScore W2201254124C186060115 @default.
• W2201254124 hasConceptScore W2201254124C2776032975 @default.
• W2201254124 hasConceptScore W2201254124C2778470358 @default.
• W2201254124 hasConceptScore W2201254124C41008148 @default.
• W2201254124 hasConceptScore W2201254124C43617362 @default.
• W2201254124 hasConceptScore W2201254124C519063684 @default.
• W2201254124 hasConceptScore W2201254124C55493867 @default.
• W2201254124 hasConceptScore W2201254124C61174792 @default.
• W2201254124 hasConceptScore W2201254124C86803240 @default.
• W2201254124 hasConceptScore W2201254124C91881484 @default.
• W2201254124 hasConceptScore W2201254124C98539663 @default.
• W2201254124 hasLocation W22012541241 @default.
• W2201254124 hasOpenAccess W2201254124 @default.
• W2201254124 hasPrimaryLocation W22012541241 @default.
• W2201254124 hasRelatedWork W1984878857 @default.
• W2201254124 hasRelatedWork W1997245611 @default.
• W2201254124 hasRelatedWork W2018613461 @default.
• W2201254124 hasRelatedWork W2046998160 @default.
• W2201254124 hasRelatedWork W2050188036 @default.
• W2201254124 hasRelatedWork W2088772156 @default.
• W2201254124 hasRelatedWork W2772529147 @default.
• W2201254124 hasRelatedWork W4200309930 @default.
• W2201254124 hasRelatedWork W4253724041 @default.
• W2201254124 hasRelatedWork W83059117 @default.
• W2201254124 isParatext "false" @default.

• next