FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2201314206> ?p ?o ?g. }

• W2201314206 endingPage "21" @default.
• W2201314206 startingPage "13" @default.
• W2201314206 abstract "Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a homotetrameric enzyme involved in a key step of glycolysis, also has a role in mediating cell death under nitrosative stress. Our previous reports suggest that nitric oxide-induced intramolecular disulfide-bonding GAPDH aggregation, which occurs through oxidation of the active site cysteine (Cys-152), participates in a mechanism to account for nitric oxide-induced death signaling in some neurodegenerative/neuropsychiatric disorders. Here, we demonstrate a rescue strategy for nitric oxide-induced cell death accompanied by GAPDH aggregation in a mutant with a substitution of Cys-152 to alanine (C152A-GAPDH). Pre-incubation of purified wild-type GAPDH with C152A-GAPDH under exposure to nitric oxide inhibited wild-type GAPDH aggregation in a concentration-dependent manner in vitro. Several lines of structural analysis revealed that C152A-GAPDH extensively interfered with nitric oxide-induced GAPDH-amyloidogenesis. Overexpression of doxycycline-inducible C152A-GAPDH in SH-SY5Y neuroblastoma significantly rescued nitric oxide-induced death, concomitant with the decreased formation of GAPDH aggregates. Further, both co-immunoprecipitation assays and simulation models revealed a heterotetramer composed of one dimer each of wild-type GAPDH and C152A-GAPDH. These results suggest that the C152A-GAPDH mutant acts as a dominant-negative molecule against GAPDH aggregation via the formation of this GAPDH heterotetramer. This study may contribute to a new therapeutic approach utilizing C152A-GAPDH against brain damage in nitrosative stress-related disorders." @default.
• W2201314206 created "2016-06-24" @default.
• W2201314206 creator A5007062462 @default.
• W2201314206 creator A5010040847 @default.
• W2201314206 creator A5026866451 @default.
• W2201314206 creator A5033320644 @default.
• W2201314206 creator A5036621496 @default.
• W2201314206 creator A5043410192 @default.
• W2201314206 creator A5050579291 @default.
• W2201314206 creator A5089926742 @default.
• W2201314206 date "2016-02-01" @default.
• W2201314206 modified "2023-10-16" @default.
• W2201314206 title "Active site cysteine-null glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rescues nitric oxide-induced cell death" @default.
• W2201314206 cites W1691536622 @default.
• W2201314206 cites W1847442346 @default.
• W2201314206 cites W1864508659 @default.
• W2201314206 cites W1978105472 @default.
• W2201314206 cites W1997880257 @default.
• W2201314206 cites W2002559713 @default.
• W2201314206 cites W2016422864 @default.
• W2201314206 cites W2020119017 @default.
• W2201314206 cites W2027459042 @default.
• W2201314206 cites W2027802092 @default.
• W2201314206 cites W2030626080 @default.
• W2201314206 cites W2036563840 @default.
• W2201314206 cites W2043801825 @default.
• W2201314206 cites W2054307806 @default.
• W2201314206 cites W2054777631 @default.
• W2201314206 cites W2055905702 @default.
• W2201314206 cites W2056987849 @default.
• W2201314206 cites W2063976323 @default.
• W2201314206 cites W2066374288 @default.
• W2201314206 cites W2066877344 @default.
• W2201314206 cites W2068748609 @default.
• W2201314206 cites W2076627263 @default.
• W2201314206 cites W2096871163 @default.
• W2201314206 cites W2118522565 @default.
• W2201314206 cites W2129150800 @default.
• W2201314206 cites W2149970565 @default.
• W2201314206 cites W2164008826 @default.
• W2201314206 doi "https://doi.org/10.1016/j.niox.2015.12.005" @default.
• W2201314206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26725192" @default.
• W2201314206 hasPublicationYear "2016" @default.
• W2201314206 type Work @default.
• W2201314206 sameAs 2201314206 @default.
• W2201314206 citedByCount "21" @default.
• W2201314206 countsByYear W22013142062016 @default.
• W2201314206 countsByYear W22013142062017 @default.
• W2201314206 countsByYear W22013142062018 @default.
• W2201314206 countsByYear W22013142062019 @default.
• W2201314206 countsByYear W22013142062020 @default.
• W2201314206 countsByYear W22013142062021 @default.
• W2201314206 countsByYear W22013142062022 @default.
• W2201314206 countsByYear W22013142062023 @default.
• W2201314206 crossrefType "journal-article" @default.
• W2201314206 hasAuthorship W2201314206A5007062462 @default.
• W2201314206 hasAuthorship W2201314206A5010040847 @default.
• W2201314206 hasAuthorship W2201314206A5026866451 @default.
• W2201314206 hasAuthorship W2201314206A5033320644 @default.
• W2201314206 hasAuthorship W2201314206A5036621496 @default.
• W2201314206 hasAuthorship W2201314206A5043410192 @default.
• W2201314206 hasAuthorship W2201314206A5050579291 @default.
• W2201314206 hasAuthorship W2201314206A5089926742 @default.
• W2201314206 hasBestOaLocation W22013142061 @default.
• W2201314206 hasConcept C104292427 @default.
• W2201314206 hasConcept C104317684 @default.
• W2201314206 hasConcept C134792803 @default.
• W2201314206 hasConcept C178790620 @default.
• W2201314206 hasConcept C181199279 @default.
• W2201314206 hasConcept C185592680 @default.
• W2201314206 hasConcept C190283241 @default.
• W2201314206 hasConcept C2776317432 @default.
• W2201314206 hasConcept C2780126713 @default.
• W2201314206 hasConcept C31573885 @default.
• W2201314206 hasConcept C519581460 @default.
• W2201314206 hasConcept C55493867 @default.
• W2201314206 hasConceptScore W2201314206C104292427 @default.
• W2201314206 hasConceptScore W2201314206C104317684 @default.
• W2201314206 hasConceptScore W2201314206C134792803 @default.
• W2201314206 hasConceptScore W2201314206C178790620 @default.
• W2201314206 hasConceptScore W2201314206C181199279 @default.
• W2201314206 hasConceptScore W2201314206C185592680 @default.
• W2201314206 hasConceptScore W2201314206C190283241 @default.
• W2201314206 hasConceptScore W2201314206C2776317432 @default.
• W2201314206 hasConceptScore W2201314206C2780126713 @default.
• W2201314206 hasConceptScore W2201314206C31573885 @default.
• W2201314206 hasConceptScore W2201314206C519581460 @default.
• W2201314206 hasConceptScore W2201314206C55493867 @default.
• W2201314206 hasFunder F4320334764 @default.
• W2201314206 hasFunder F4320334789 @default.
• W2201314206 hasLocation W22013142061 @default.
• W2201314206 hasLocation W22013142062 @default.
• W2201314206 hasOpenAccess W2201314206 @default.
• W2201314206 hasPrimaryLocation W22013142061 @default.
• W2201314206 hasRelatedWork W1987470712 @default.
• W2201314206 hasRelatedWork W1988064189 @default.
• W2201314206 hasRelatedWork W2030427530 @default.
• W2201314206 hasRelatedWork W2037075372 @default.

• next