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- W2201609585 abstract "We studied the influence of 5-fluorocytosine(5FC) on the monomeric 80S ribosomes isolated by gradient ultracentrifugation from Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus and Wangiella dermatitidis. Labelling of the cultures with 32PO4 and [3H]-leucine showed marked inhibition of both ribosomal RNA and ribosomal protein synthesized during the contact of the fungi with the drug. The degree of inhibition was very similar for the synthesis of both ribosomal constituents, (mostly 60–80% each during the initial hours of the contact). This indicates that some ribosomes were still synthesized in the presence of 5FC and that the drug inhibits the synthesis of complete ribosomes to the same extent as the synthesis of ribosomal RNA and protein. By labelling fungi with [14C]-5FC it was shown that in those 80S ribosomes that were still synthesized, large quantities of the drug were incorporated—doubtless as 5-fluorouracil (5FU) in RNA. It can be concluded that RNA containing 5FU is still capable of combining with protein to form complete ribosomes of normal conformation. This is, however, no proof that these ribosomes would have a normal function. Bei Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus und Wangiella dermatitidis wurde die Wirkung von 5FC auf monomere mittels Gradienten-Ultrazentrifugierung isolierten 80S Ribosomen untersucht. Markierung mit 32PO4, und [3H]-Leucin zeigte eine starke Hemmung der ribosomalen RNS und Proteinsynthese unter Einfluss von 5FC. Die Hemmung war, besonders während des ersten Kontakts mit 5FC, gleich stark für beide ribosomalen Aufbausteine (Protein und RNS), namlich 60–80%. Dieses Resultat beweist, dass die Konformation der Ribosomen unter 5FC wahrscheinlich normal ist, und dass 5FC die Synthese von 80S Ribosomen hemmt. Durch Einbau von radioaktivem 5FC wurde gezeigt, dass in die 80S Ribosomen grosse Mengen von 5FC eingebaut wurden (naturlich in Form von 5FU in die RNS). Daraus kann geschlossen werden, dass die mit 5FU modifizierte RNS sich immer noch Proteinem kombiniert um komplette Ribosomen zu bilden. Es besteht aber kein Beweis, dass diese Ribosomen noch eine normale Funktion haben." @default.
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- W2201609585 date "1981-01-01" @default.
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- W2201609585 title "The effect of 5-fluorocytosine on the synthesis of 80S ribosomes by pathogenic fungi" @default.
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- W2201609585 doi "https://doi.org/10.1080/00362178185380301" @default.
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